The discovery and engineering of novel fluorescent proteins (FPs) from diverse organisms is yielding fluorophores with exceptional characteristics for live‐cell imaging. In particular, the development of FPs for fluorescence (or Förster) resonance energy transfer (FRET) microscopy is providing important tools for monitoring dynamic protein interactions inside living cells. The increased interest in FRET microscopy has driven the development of many different methods to measure FRET. However, the interpretation of FRET measurements is complicated by several factors including the high fluorescence background, (...) the potential for photoconversion artifacts and the relatively low dynamic range afforded by this technique. Here, we describe the advantages and disadvantages of four methods commonly used in FRET microscopy. We then discuss the selection of FPs for the different FRET methods, identifying the most useful FP candidates for FRET microscopy. The recent success in expanding the FP color palette offers the opportunity to explore new FRET pairs. (shrink)

This very short introduction will first outline how ontological investigations and questions of practice go together. The second section will bring in the next pole of this entire book, history of mathematics. How do ontology, practice, and history go together? Is this a forced marriage or one born in true love? That is, do these three belong together in some very basic way? One chapter in the section argues that the philosophy of mathematical practice intersects with the history of mathematics (...) essentially. If the first section of this introduction is persuasive that will also show that the history of mathematics intersects with ontological questions as well, specifically, that historical questions are very much about the ontological commitments of past mathematicians. The last section will take up what might seem a very different theme, namely, how these things matter to mathematics education. This section is connected closely to the second section since mathematics education has taken an interest in the role of history of mathematics in mathematics education, and much insight can be gained by seeing the issues in relation to the ontology-practice-history triad. The question of education is also important since teachers must consider what it is exactly that they are teaching, or, to adapt the title of Reuben Hersh’s well-known book, “What is mathematics really about?” And the “aboutness” of mathematics is really the ontological question. (shrink)

New brain-computer interface and neuroimaging techniques are making differentiation less ambiguous and more accurate between unresponsive wakefulness syndrome patients and patients with higher cognitive function and awareness. As research into these areas continues to progress, new ethical issues will face physicians of patients suffering from total locked-in syndrome, characterized by complete loss of voluntary muscle control, with retention of cognitive function and awareness detectable only with neuroimaging and brain-computer interfaces. Physicians, researchers, ethicists and hospital ethics committees should be aware of (...) and prepared to handle ethical issues unique to these totally locked-in patients. Several thought experiments are discussed, to highlight potential ethical dilemmas surrounding surrogate decision-making, autonomy, end-of-life care, and pediatric care, which will be unique to total LIS patients. These, along with other ethical problems especially relevant to total LIS patients, merit further discussion among physicians, researchers, ethicists and hospital ethics committees, to facilitate consensus regarding these issues, and improve patient care. (shrink)

There are at least twelve major virtues of good theories: evidential accuracy, causal adequacy, explanatory depth, internal consistency, internal coherence, universal coherence, beauty, simplicity, unification, durability, fruitfulness, and applicability. These virtues are best classified into four classes: evidential, coherential, aesthetic, and diachronic. Each virtue class contains at least three virtues that sequentially follow a repeating pattern of progressive disclosure and expansion. Systematizing the theoretical virtues in this manner clarifies each virtue and suggests how they might have a coordinated and cumulative (...) role in theory formation and evaluation across the disciplines—with allowance for discipline specific modification. An informal and flexible logic of theory choice is in the making here. Evidential accuracy, according to my systematization, is not a largely isolated trait of good theories, as some have made it out to be. Rather, it bears multifaceted relationships, constituting significant epistemic entanglements, with other theoretical virtues. (shrink)

BackgroundDespite severe cognitive dysfunction in Alzheimer's disease, aesthetic preferences in AD patients seem to retain some stability over time, similarly to healthy controls. However, the underlying mechanisms of aesthetic preference stability in AD remain unclear. We therefore aimed to study the role of emotional valence of stimuli for stability of aesthetic preferences in patients with AD compared to cognitively unimpaired elderly adults.MethodsFifteen AD patients score 12–26) without visual impairment and/or psychiatric disorder, as well as 15 healthy controls without cognitive impairment (...) matched in age, sex, art interest and highest level of education were included in this study. All participants were asked to rank-order eight artworks per stimulus category according to their preference twice with a 2-week span in-between. Based on these two rankings a preference change score was calculated. In order to assess explicit recognition memory of the artworks in the second testing session, four artworks of each stimulus category used in the preference ranking task were presented together with a content-matched distractor artwork painted by the same artist. Participants had to indicate which of the stimuli they had seen 2 weeks previously.ResultsAD patients [MMSE = 18.9 ± 3.6; Age = 85.4 ± 6.9; 33.3% male] had no explicit recognition memory of the artworks, whereas healthy controls [MMSE = 27.7 ± 1.4; Age = 84.3 ± 6.7; 33.3% male] correctly recognized 85% of stimuli after 2 weeks. AD patients had equally stable preferences compared to the control group for negative artworks, but less stable preferences for positive and neutral images.ConclusionEven in cognitively impaired AD patients, aesthetic preference for negatively-valenced artworks remains relatively stable. Our study provides novel evidence that AD patients may have a somewhat preserved implicit valence system for negative compared to neutral or positive visual information, especially in the domain of aesthetics. However, more studies need to further uncover the details of the underlying neurocognitive mechanisms of preference stability in pathological aging. (shrink)

The onset of the electronics-based information revolution will augur changes in the sociological perceptions of 'suitable careers' for women. This phenomenon is particularly evident in rural Appalachia. A planned, systematic delivery system was designed, developed, and implemented by Southwest Virginia Community College to introduce women to the challenges and possibilities of technical careers. This was accomplished through a gradualized phase-in to Technological Literacy, followed by in-depth involvement, culminating in an industrial internship experience. A special curriculum was designed to ease the (...) transition of the women, most of whom were homemakers, into the fast-paced mainstream of the academic environment. Duration of the introductory segment was one academic quarter. All project participants were clustered in a peer group, led by the project director, a trained counselor. In addition to a central course on Career Directions for Women, the program design emphasized Industrial Dynamics, the variety of uses possible with "user-friendly" microcomputers, Computer-Assisted Drafting, Electronics Instrumentation, Mathematics and Communications. Field experiences and visits to area industries were integrated into the program plan, as were visits from women who had achieved success in technical careers. Particular efforts were made to arrange for visits by the limited number of women alumni from the college's technical programs. Students were then mainstreamed into a regular program in Electronics or Computer Aided Drafting in the intermediate segment. Students are given a closer look at the industrial sector in the final segment. An examination of the social structure in Appalachian Virginia is presented, together with an albeit tentative prediction of the implications for female wage earners in this highly traditional region, so dependent heretofore on coal mining. Finally, the concept of whether this effort will lead to evolution, rather than revolution, is discussed. While covering a somewhat homogeneous and unique population cluster, the action blueprint outlined can be applied to other population groups in the U.S. and abroad. (shrink)

Der Titel meines Vortrags bezieht sich nicht auf heftige Auseinandersetzungen in der heutigen Hegelrezeption, sondern auf den gleichnamigen Abschnitt der Phänomenologie des Geistes von 1807: “Das geistige Tierreich und der Betrug oder die Sache selbst.” Dieser verhältnismäßig wenig beachtete und womöglich noch weniger verstandene Abschnitt ist meines Erachtens einer der wichtigsten im ganzen Buch. Ich möchte deshalb heute versuchen seine Bedeutung etwas aufzuklären.

This article is concerned with the nature of Socratic refutation.Over fifty years agon ow, Richard Robinson argued that Plato‘s Socrates assumes that his refutations show an interlocutor‘s thesis, not merely to contradict other beliefs held by the interlocutor, but to be self-contradictory. At first sight,this interpretation does not seem plausible, and it would indeed be rejected by most scholars today. Nevertheless, I argue that the interpretation contains much truth: Plato‘s Socrates does, if not always, then at least sometimes think of (...) his refutations in this way. In addition – and here I part company not only with Robinson‘s critics but also with Robinson himself – I argue for two further claims: first,that in thinking of his refutations in this way Socrates gives a reasonable characterization of a significant number of his refutations; and second, that it is not implausible of him to suppose that theses of the sort that he attempts to refute are in fact self-contradictory. (shrink)

Consideration of the German philosophy and political history of the past century might well give the impression, and often does give foreign observers the impression, that liberalism, including in particular commitment to the ideal of free thought and expression, is only skin-deep in Germany. Were not Heidegger's disgust at Gerede (which of course really meant the free speech of the Weimar Republic) and Gadamer's defense of "prejudice" and "tradition" more reflective of the true instincts of German philosophy than, say, the (...) Frankfurt School's heavily Anglophone-influenced championing of free thought and expression? Were not the Kaiser and Nazism more telling of Germany's real political nature than the liberalism of the Weimar Republic (a desperate, ephemeral experiment undertaken in reaction to Germany's disastrous defeat in World War I) or the liberalism of (West) Germany since 1945 (in effect forced on the country by the victorious Allies after World War II)? (shrink)

Barnett argues that the United Nations, by operating on the principle of the consent of the parties, can encourage the development of a more stable and cooperative security architecture.

In the future, human destiny may depend on our ethics. In particular, biotechnology and expansion in space can transform life, raising profound questions. Guidance may be found in Life-centered ethics, as biotic ethics that value the basic patterns of organic gene/protein life, and as panbiotic ethics that always seek to expand life. These life-centered principles can be based on scientific insights into the unique place of life in nature, and the biological unity of all life. Belonging to life then implies (...) a human purpose: to safeguard and propagate life. Expansion in space will advance this purpose but will also raise basic questions. Should we expand all life or only intelligent life? Should we aim to create populations of trillions? Should we seed other solar systems? How far can we change but still preserve the human species, and life itself? The future of all life may be in our hands, and it can depend on our guiding ethics whether life will fulfil its full potentials. Given such profound powers, life-centered ethics can best secure future generations. Our descendants may then understand nature more deeply, and seek to extend life indefinitely. In that future, our human existence can find a cosmic purpose. (shrink)

Discrediting 'mystical' or 'psychical' interpretations of out-of-body and near-death experiences, Michael Marsh demonstrates how these phenomena are explicable in terms of brain neurophysiology and its neuropathological disturbances, and discusses the theological and philosophical implications of his hypotheses.

This paper surveys central justifications and approaches adopted by educators interested in incorporating history of mathematics into mathematics teaching and learning. This interest itself has historical roots and different historical manifestations; these roots are examined as well in the paper. The paper also asks what it means for history of mathematics to be treated as genuine historical knowledge rather than a tool for teaching other kinds of mathematical knowledge. If, however, history of mathematics is not subordinated to the ideas and (...) methods at the heart of the usual mathematical curriculum – algebraic equations, functions, derivatives, analytic geometry – if it becomes an essential subject for mathematics, then the attempt to find a place for history of mathematics requires refining our understanding of the nature of mathematics education itself. Thus, the paper asks not only how can history of mathematics be incorporated into mathematics education but also how the idea of mathematics education may need to be adjusted to accommodate history. (shrink)

This exploratory study investigated Catholic priests' knowledge and perceptions of pastoral codes of conduct and their perceptions about the processes for reporting misconduct. Overall, respondents understood that they had to breach confidentiality when parishioners divulged a threat to harm self or others or when there was an allegation of misconduct involving a colleague. Fewer respondents understood that information received in spiritual counseling or spiritual direction must be maintained confidentially. Respondents were aware that their codes of pastoral conduct offered guidance about (...) engaging in dual relationships (i.e., friendships with parishioners), but most respondents included parishioners in their circles of friends. (shrink)

This chapter examines certain claims made for near‐death and out‐of‐body experiences (ND/OBE), adding neuro‐physiological and theological insights. ND/OBE aredecidedly this‐worldly events and have nothing to do with supposed journeys to spiritualized or nonphysical realms, nor amalgamations with so‐called cosmic consciousness. Classical spiritual encounters were discussed by William James, and by William P. Alston. The chapter compares classic examples of divine disclosure with those given by NDE subjects. Considering the “spiritual” properties of NDE reports, one might be somewhat reluctant to credit (...) them with any serious divine import, or, like some of the otherreports, of enlightening revelatory content. The chapter also summarizes author's analysis that offers arguments against any serious import to NDE phenomenology, other than deriving from stored material in brains of subjects reporting. It continues in critically examining the notion of afterlife, as envisioned throughout the NDE literature, compared with Christian precepts of the creedal “Life Everlasting”. (shrink)

Argument“The problem of university courses on infinitesimal calculus and their demarcation from infinitesimal calculus in high schools” is the published version of an address Otto Toeplitz delivered at a meeting of the German Mathematical Society held in Düsseldorf in 1926. It contains the most detailed exposition of Toeplitz's ideas about mathematics education, particularly his thinking about the role of the history of mathematics in mathematics education, which he called the “genetic method” to teaching mathematics. The tensions and assumptions about mathematics, (...) history of mathematics, and historiography revealed in this piece dedicated to educational ideas are what make Toeplitz's text interesting in the study of historiography of mathematics. In general, the ways historiography of mathematics and teaching of mathematics, even without an immediate concern for history, are deeply entangled and, in our view, worth attention both in historical and educational research. (shrink)

Prehistoric archaeology, which in the American academic structure is part of anthropology, has always included and continues to include the study of social aspects of technology, particularly of technological innovation. Despite early calls for their inclusion in the field of science, technology, and society, however, archaeologists and their research have not, by and large, been integrated into this new discipline. This article is a renewed appeal for the use of archaeology in studying issues of technology and society. An example drawn (...) from prehistoric Europe illustrates how archaeology can provide a fresh perspective on social aspects of technological innovation, as well as specific data for cross-cultural comparison. (shrink)

Men who spend a greater proportion of time apart from their female partner since the couple’s last copulation are at greater “objective” sperm competition risk. We propose a novel cue to sperm competition risk: the time she spends with her male friends. Four hundred and twenty men in a committed, heterosexual, sexual relationship completed a questionnaire. The results indicate that men at greater objective sperm competition risk report less time desired until the couple’s next copulation, greater interest in copulating with (...) their partner, and greater anger, frustration, and upset in response to their partner’s sexual rejection, but only among men whose partner spends more time with her male friends. These results remain after controlling statistically for the participant’s age and their partner’s age. We discuss limitations of the current research, and discuss how research in human sperm competition can inform social issues, including men’s partner-directed sexual coercion. (shrink)

Whitehouse's model explains when people engage in self-sacrifice, but not who is most likely to do so. We propose incorporating individual differences, such as cognitive style (one's inclination toward intuition versus deliberation), and argue that individuals who rely on intuition may be more likely to (1) develop group identity fusion after an emotional experience and (2) engage in pro-social self-sacrifice.