Two reports have shown that mammalian artificial chromosomes (MAC) can be constructed from cloned human centromere DNA and telomere repeats, proving the principle that chromosomes can form from naked DNA molecules transfected into human cells. The MACs were mitotically stable, low copy number and bound antibodies associated with active centromeres. As a step toward second-generation MACs, yeast and bacterial cloning systems will have to be adapted to achieve large MAC constructs having a centromere, two telomeres, and genomic copies of mammalian (...) genes. Available construction techniques are discussed along with a new P1 artificial chromosome (PAC)-derived telomere vector (pTAT) that can be joined to other PACs in vitro, avoiding a cloning step during which large repetitive arrays often rearrange. The PAC system can be used as a route to further define the optimal DNA elements required for efficient MAC formation, to investigate the expression of genes on MACs, and possibly to develop efficient MAC-delivery protocols. BioEssays 1999;21:76–83. © 1999 John Wiley & Sons, Inc. (shrink)

This book addresses the idea of justice in order to guide society towards a more effective justice system. The authors trace impoverished and accomplished thinking in criminological and justice discourses and show that when justice and love are seen as synonyms, the historic ills that have plagued humanity tend to evaporate.

Shavelson and colleagues (2023) describe how medical aid-in-dying laws in the United States prohibit assistance in administering aid-in-dying medication. This prohibition distinguishes aid in dying...

Persons with dementia often prefer to participate in decisions about their health care, but may be prevented from doing so because healthcare decision-making law facilitates use of advance directives or surrogate decision makers for persons with decisional impairments such as dementia. Federal and state disability law provide alternative decision-making models that do not prevent persons with mild to moderate dementia from making their own healthcare decisions at the time the decision needs to be made. In order to better promote autonomy (...) and wellbeing, persons with dementia should be accommodated and supported so they can make their own healthcare decisions. (shrink)

Discussion of supported decision making has been dominated by legal scholars, philosophers, and advocates for persons with disabilities. Peterson et al.’s primary contribution is introducing...

“The Bodhisattva’s Practice of Enlightenment” interprets a Los Angeles Times newspaper opinion piece by Thich Nhat Hanh as a contemporary image of enlightenment with far-reaching implications. Written in response to the brutal police beating of Rodney King and not intended for a Buddhist audience, this brief newspaper article nevertheless provides glimpses into the contemporary meaning of enlightenment. The chapter considers Thich Nhat Hanh’s comments in relation to the basic principles of Buddhist ethics before turning to the Vimalakīrti sūtra, a classical (...) Mahayana Buddhist scripture, to extend an understanding of what it would mean to experience the world through an in-depth awareness of “no-self.” Dwelling on the bodhisattva’s effort to cultivate generosity of spirit, the chapter considers the kinds of human relations that Thich Nhat Hanh’s sense of enlightenment entails. (shrink)

Buddhism: What Everyone Needs to Know offers readers a brief, authoritative guide to one of the world's largest and most diverse religious traditions in a reader-friendly question-and-answer format. Dale Wright covers the origins and early history of Buddhism, the diversity of types of Buddhism throughout history, and the status of contemporary Buddhism. This is a go-to book for anyone seeking a basic understanding of the origins, history, teachings, and practices of Buddhism.

Background:The severity of the Coronavirus pandemic has led to lockdowns in different countries to reduce the spread of the infection. These lockdown restrictions are likely to be detrimental to mental health and well-being in adolescents. Physical activity can be beneficial for mental health and well-being; however, research has yet to examine associations between adolescent physical activity and mental health and well-being during lockdown.Purpose:Examine the effects of adolescent perceived Coronavirus prevalence and fear on mental health and well-being and investigate the extent (...) to which physical activity can be a protective factor against these concerns.Methods:During United Kingdom lockdown restrictions, 165 participants (100 female, aged 13–19) completed an online questionnaire assessing perceived Coronavirus prevalence and fear, physical activity, and indicators of mental health and well-being (stress, anxiety, depression, fatigue, vitality, and perceived health). Separate hierarchical multiple linear regression analyses (with age, gender, perceived Coronavirus prevalence, and fear entered in step 1, and physical activity in step 2) were run to predict each well-being outcome.Results:Regression analyses indicated that in general, while Coronavirus fear was a negative predictor, physical activity was a positive and stronger predictor of enhanced mental health and well-being outcomes.Conclusion:Findings suggest that physical activity during the Coronavirus pandemic can counteract the negative effects of Coronavirus fear on adolescent mental health and well-being. Therefore, physical activity should be promoted during lockdown to support good mental health and well-being. (shrink)

Hua-yen Buddhism, the pre-eminent philosophical form of Buddhism in early T'ang dynasty the China, was instrumental in laying the conceptual foundations for virtually all subsequent East Asian Buddhism. This Hua-yen legacy includes Ch' an/Zen and Pure Land, the non-philosophical forms of Buddhism that came to dominance in the centuries to follow. In this sense, Fa-tsang (643-712), the third patriarch and foremost philosopher of Hua-yen, can be considered one of the forefathers of East Asian Buddhism today. By focusing on one element (...) in Fa-tsang's thought, this essay attempts to articulate the overall character of Hua-yen thought and, in the process, to shed light on its connection to other dimensions of the Buddhist tradition. -/- In Fa-tsang's monumental Wu-chiao chang (Treatise on the Five Teachings ), the mental image of a house is taken to model the dharmakãya universe as a whole. On Fa-tsang's account, since any one part of the house - his example is a rafter - is a condition for the house as a whole, that one part through its complex relations encompasses the whole house and is therefore able to reveal it comprehensively. What he calls the "one flavor" of the dharmakãya can be fully tasted in any one part. Adopting Fa-tsang's sytematic principle and taking the concept of bodhicitta as the focal point study, this essay attempts to show the "one flavor" of Fa-tsang's Buddhist thought through the treatment he gives to the traditional idea of the "thought of enlightenment.". (shrink)

Incapacitated adults with a legally appointed guardian or conservator may be recruited for or involved with medical, behavioral, or social science research. Much of the research in which such persons participate is aimed at evaluating medical interventions for them, or contributing to general knowledge about disorders from which they may suffer. In this paper we will consider how the appointment of guardians for patients with disorders of consciousness —severe brain injuries that affect a patient’s level of arousal and ability to (...) interact —impacts such patients’ access to research.Such persons may be under guardianship because they have lost decisional capacity (i.e., those in a coma or... (shrink)

Pickering, Newton-Howes, and Young assert that the traditional view of decisional capacity, premised on assessing patients’ abilities to communicate, understand, appreciate,...

relphiPhilosophy of ReligionThat neither Buddhist philosophy nor contemporary standards of thinking would justify Buddhists today continuing to assume, as many traditional Buddhists have, that enlightenment is a preexistent human ideal that is fixed and unchanging for all human beings in all times. Even though Buddhism was founded on the profound realization that all things are impermanent and interdependent, faith in a fixed and independent human nature and corresponding to that nature an unchanging enlightened ideal for life have been maintained throughout (...) much of the Buddhist tradition. We can certainly understand and admire the extent to which the tradition took the image of the Buddha’s enlightenment as the standard against which all others would be measured and on which all forms of human excellence would be modeled. But that timeless conception of enlightenment can no longer be underwritten for us either by traditional Buddhist narratives or by a static conception of human nature. As human practices, capacities, needs, and interests change, so will the images of human excellence that we come to admire and pursue in our lives.... (shrink)

“Language in Zen Enlightenment” considers the role language might play in the experience of enlightenment. Building on the Zen claims that enlightenment is “not dependent on language and culture” and that enlightenment is a “pure experience” of “things as they are” prior to the shaping effect of language, this chapter takes the perspective of contemporary philosophy and linguistic psychology in order to assess the two primary Western interpretations of the relation between language and Zen enlightenment. To articulate an alternative understanding (...) of language and spiritual experience that goes beyond these earlier attempts, the chapter explores the kinds of language use found in the daily life of Zen communities, the role of the Zen master’s cryptic rhetoric of instruction, and the predominance of silent meditation in Zen training. It concludes with reflection on the possibility that Zen enlightenment might entail awakening to the power and subtlety of language rather than from it. (shrink)

Canada has a marked shortfall between the supply and demand for kidneys for transplantation. Median wait times for deceased donor kidney transplantation vary from 5.8 years in British Columbia, 5.2 years in Manitoba and 4.5 years in Ontario to a little over 2 years in Quebec and Nova Scotia. Living donation provides a viable option for some, but not all people. Consequently, a small number of people travel abroad to undergo kidney transplantation by commercial means. The extent to which they (...) are aware of the potential risks to their health and the health of the kidney vendors is unclear. Travel abroad to obtain a kidney commercially i.e. transplant tourism (TT), raises ethical issues which include the exploitation of the poor, uncertainty of donor informed consent to nephrectomy, poor clinical care and lack of follow up for the donor, commodification of the body and inequity of access to medical care for donors. Also, TT widens socioeconomic disparities in access to transplantation, differing from the Canadian system of universal coverage for healthcare. The Canadian transplant community has discussed how to respond to patients who plan to travel abroad for TT or return with a purchased kidney. Unease rests in the tension between the duty to care for legitimate Canadian residents and the unwillingness to enable TT. This paper discusses three anonymized cases and the Canadian responses to TT as recorded in academic literature and a formal statement by relevant professional bodies. (shrink)

“Enlightenment and the Practice of Meditative Reading” examines the practices of reading and the critiques of reading that were common in the first few centuries of Chinese Chan Buddhist monasticism. Although interpreters frequently conclude that reading ceased to be a central practice in the institutions of Chan, classical texts show that, reading and writing were just as central to Chan practice as they had been in earlier forms of Chinese Buddhism. Because China’s culture of literacy inevitably extended into Chan practice, (...) a highly unusual and sophisticated theory of reading developed, while practices of reading aimed at enlightenment thrived. This historical realization raises questions about the relation between practices of meditation and reading, suggesting that our own practice of reading might be developed and deepened by learning to conceive of it as a form of meditative practice. (shrink)

“From the Thought of Enlightenment to the Event of Awakening” follows the philosophical reflections of Fazang of the Huayan school of Chinese Buddhism as he explores the progression along the Buddhist path from an initial concept or image of what enlightenment might be all the way through to the culminating experience of enlightenment. His paradoxical claim that complete enlightenment is already fully contained in the first legitimate thought of enlightenment is analyzed in this chapter by understanding it in relation to (...) the basic components of the Huayan Buddhist worldview of thoroughgoing interdependence. Fazang’s Huayan Buddhist philosophy includes in an early philosophical form the basic conceptual elements of the historic debate in China concerning the experience of enlightenment—the widely cited controversy about whether enlightenment occurs gradually over time or through a sudden, momentous breakthrough. This chapter explores the enlightening consequences of meditation on the interdependent character of all realities. (shrink)

“Secular Buddhism and the Religious Dimension of Enlightenment” follows Stephen Batchelor’s impressive effort to articulate what Buddhist awakening might mean in the current secular culture. Assessing the issues that have prompted the creation of a new nonsectarian “secular Buddhism,” this chapter raises questions about whether this form of secularity nevertheless continues to carry religious meaning as its most fundamental motivation. Reflecting on the possibility of nontheistic forms of religious practice and experience, it links insights in contemporary Western religious thought to (...) the a-theism that runs throughout the Buddhist tradition in order to further inquiry into the qualities and character that Buddhist enlightenment might take under contemporary, secularized conditions. The chapter aspires to cultivate insight into the inevitable relationship between religiosity and any quest for enlightenment and aims to stimulate reflection on what religion might be in our time. (shrink)

Today’s unstable economy puts added stress on today’s leaders. When these leaders are stressed, maintaining their values becomes more difficult. While ethics are stressed during one’s education, organizations are less likely to continue ethics training. Given the many scandals, it is essential that there are ethical leaders to guide organizations. A leader cannot be prepared for all situations, but an ethical leader will have the tools to guide himself through complex situations. The purpose of this article is to present how (...) ethics training is often lacking once students move to the business world. However, continuous training is needed to reinforce their skills throughout their careers. This article will use a review of literature to present findings in academia and business. (shrink)

McCarthy and Howard (2023) advocate for supported decision making for patients with intellectual and developmental disabilities (IDD). Departing from the “mental prosthesis” model of supported deci...

relphiPhilosophy of ReligionWHEN EUROPEAN INTELLECTUALS in the late eighteenth century engaged in high-profile, public debate on the question “What is enlightenment?,”1 Buddhist answers to this question never came up for consideration, and it is safe to say that no participant in these debates would have or could have believed that this “oriental” religion had anything even remotely to do with “enlightenment.” Although knowledge of Buddhism was already accumulating in Europe by that time through the merchants, missionaries, and soldiers that the (...) various European empires had dispatched to Asia, what they “knew” about Buddhism was that this ascetic, monastic religion was curiously nihilistic in its central goal:... (shrink)

“The Awakening of Character as an Image of Enlightenment” describes in some detail two unforgettable characters in the Korean Buddhist film Mandala in order to consider the range of diversity that enlightenment can encompass. Two very different Buddhist monks are presented in the film as both deeply enveloped in ordinary human suffering and as breaking through that suffering to experience a transformation of character that we can only understand as enlightenment. While the kinds of character that emerge in each case (...) are quite distinct, viewers are left to puzzle over how these entirely different paths through human life could both be legitimate instances of enlightenment. While each character in the film provides an image of the Buddhist path, readers are presented with the task of sorting out how their own paths align or contrast with the two provided. (shrink)

“The Thought of Enlightenment and the Dilemma of Human Achievement” examines the Buddhist concept of bodhicitta—the thought of or aspiration for enlightenment. The chapter compares this Buddhist concept to the Greek philosophical notion of the “idea of the good” and finds both playing similar roles in articulating ideal ends—the ultimate goals of human life. If in actual human life, however, these ideals are never fully achieved, a dilemma emerges at the heart of human practice. Although the transformations cultivated through practice (...) may be substantial and far-reaching, perfection remains always beyond out of reach. This chapter examines several reasons for that inevitable unreachability and concludes that, rather than undermining the value or legitimacy of the quest, this dilemma of human achievement adds nuance and depth to the meaning of human enlightenment. Because human beings will always strive toward some form of excellence, humility and the capacity to release the tension of compulsive perfectionism become essential features of enlightenment. (shrink)

Although the Federal Common Rule requires that informed consent documents include all material information, it does not specify the content of materials used to recruit human subjects. In particular, there is no federal regulation relating to how payment for research participation is to be advertised. Rather, the FDA has issued guidance, advising researchers not to emphasize payment information. In order to determine how IRBs have interpreted this guidance, we coded the policies of the top 100 institutions by receipt of NIH (...) funding, in order to determine whether they require, permit, or forbid researchers to disclose the amount of compensation in their recruitment materials. We found that the vast majority of institutions implicitly or explicitly permit such disclosures; however, there are a significant number of IRBs at each extreme, some discouraging or forbidding with others encouraging or mandating such disclosures. Such heterogeneity in local regulations suggests that IRB discretion may be imposing costs on human subjects and the scientific enterprise that outweigh the benefits. We suggest that this heterogeneity should be resolved towards a national consensus on permissibility. (shrink)

This paper analyzes the kind of historical understanding presupposed in the writing of classical Chinese Ch'an Buddhist "transmission" narratives and places this historical understanding into comparative juxtaposition with modern Western historiographic practice. It finds that fundamental to Chinese Ch'an historical awareness are genealogical metaphors structuring historical time and meaning in terms of generations of family relations and the practices of inheritance. These metaphors link the Ch'an historian to the texts of historical study in ways that contrast with the posture of (...) modern historians. The essay outlines four basic differences between the self-understanding presupposed in Ch'an Buddhist historical writing and that assumed in modern historical research and concludes by suggesting how contemporary historical thinking might benefit from reflection on these differences. (shrink)

Pullman (2023) compares medically-assisted dying (MAiD) laws and rates of medically-assisted deaths in Canada and California, noting some differences in the legal regime and a higher rate of MAiD i...

The essays in this collection treat historical, literary, and philosophical topics related to Ayn Rand's Anthem, an anti-utopia fantasy set in the future. The first book-length study on Anthem, this collection covers subjects such as free will, political freedom, and the connection between freedom and individual thought and privacy.

Here, Bob Hale and Crispin Wright assemble the key writings that lead to their distinctive neo-Fregean approach to the philosophy of mathematics. In addition to fourteen previously published papers, the volume features a new paper on the Julius Caesar problem; a substantial new introduction mapping out the program and the contributions made to it by the various papers; a section explaining which issues most require further attention; and bibliographies of references and further useful sources. It will be recognized as (...) the most powerful presentation yet of a neo-Fregean program. (shrink)

This is the first scholarly study of Atlas Shrugged, covering in detail the historical, literary, and philosophical aspects of Ayn Rand's magnum opus. Topics explored in depth include the history behind the novel's creation, publication, and reception; its nature as a romantic novel; and its presentation of a radical new philosophy.