The key role played by online platforms in the neo-intermediation of the public debate requires a review of current tools for mapping the digital information ecosystem, highlighting the political nature of such an analysis: Starting from a synoptic overview of the main models of platform governance, we try to understand whether the ongoing European shift towards the Limited Government Regulation (LGR) model will be able to counterbalance the “systemic opinion power” of the giant platforms and restore the “health” of the (...) digital information ecosystem. A close analysis of the European Digital Services Act (DSA) has highlighted some limitations in achieving its goals, because of the features of the LGR on the one hand, and the disruptive features of the algorithmic neo-intermediation phenomenon on the other. Thus, we suggest a tripartite regulatory model, that can be defined as “neo-editorial accountability.” However, increasing users’ critical algorithmic awareness is an essential prerequisite for implementing the suggested template, and mitigating an outstanding effect of the LGR model: the normalization of the ideological assumptions underlying informational capitalism. (shrink)

The complexity of our ‘object’ of study, leading to the question ‘what is really the city?’ requires the use of different levels of analyses. At the same time, a way must be found to develop an explanatory model that brings together the knowledge thus produced. The study of governance in the city is necessarily part of any research aimed at investigating empirically the processes regulating the social life. The key implication is to address its impact; a task made particularly complex (...) by the often difficult relationship between the implementation of governance and the interaction with a diversity of societal actors, for the ongoing dialectic between the formal and the informal in urban life. We often find that this dialectic produces ambiguity, unexpected results, gaps between planned objectives, and competition instead of cooperation among actors, pointing to the weakness of the rationalistic model of public action. Perhaps the most important lesson that we have learnt about government and governance is that the delusion that we feel when trying to understand urban policies results from our incapacity fully to grasp the complexity of human life. (shrink)

Diogenes, Ahead of Print. The complexity of our ‘object’ of study, leading to the question ‘what is really the city?’ requires the use of different levels of analyses. At the same time, a way must be found to develop an explanatory model that brings together the knowledge thus produced. The study of governance in the city is necessarily part of any research aimed at investigating empirically the processes regulating the social life. The key implication is to address its impact; a (...) task made particularly complex by the often difficult relationship between the implementation of governance and the interaction with a diversity of societal actors, for the ongoing dialectic between the formal and the informal in urban life. We often find that this dialectic produces ambiguity, unexpected results, gaps between planned objectives, and competition instead of cooperation among actors, pointing to the weakness of the rationalistic model of public action. Perhaps the most important lesson that we have learnt about government and governance is that the delusion that we feel when trying to understand urban policies results from our incapacity fully to grasp the complexity of human life. (shrink)

This article asks whether a myth can be collapsed into history by describing the material signs of memory as "evidence." In a story dear to the Venetian hearts, in 1177 the Republic of Venice defeated the imperial fleet and brought peace back to Italy. For centuries, this narrative was recounted and its truth upheld against growing political and scholarly criticism, first through the ritual display of evidence, then through its discussion in a large literary production. In the seventeenth century, it (...) is argued, the notion of evidence was abandoned in favor of the sheer affirmation of power. (shrink)

In vitro experimental approaches are of central importance to contemporary molecular and cellular biology and toxicology. However, the scientific value or impact of in vitro results depends on their relevance in vivo. In vitro effect studies address inobservable in vivo phenomena through experiments on analogous in vitro phenomena. We present a theoretical basis developed to evaluate the in vivo relevance of in vitro effect studies. As a case study, the procedure for measuring specific gene transcription in isolated (...) cell nuclei (nuclear run-off method) is analyzed It is concluded that current evidence fails to justify in vivo interpretations of nuclear run-off experiments within the framework of theoretical models of transcription, implying that quantitative in vivo interpretations are unwarranted. Qualitative interpretations of nuclear run-off experiments may be justified by inferring the best explanation, especially when significant in vitro effects follow in vivo perturbations.Elements of a general theory are proposed. It is concluded that quantitative in vivo interpretations are warranted primarily in biochemical quantitation of biomolecules, while studies on biological function should be interpreted qualitatively in terms of causal explanations. Inferences to the best explanations are strengthened through additional evidence and the creation of experimental differences (effects). (shrink)

In vivo explore des questions fondamentales et des moments cruciaux de l’existence humaine – l’entrée en interaction avec une culture étrangère, la décision de se sortir d’une condition de vie routinière ou malheureuse, une action généreuse posée dans un contexte quotidien ordinaire – en fonction de leur potentiel de transformation de l’existence. En recourant à des illustrations tirées de la vie réelle et d’œuvres de fiction, Gabor Csepregi révèle le rôle primordial des sentiments personnels dans le façonnement de la (...) vie humaine et démontre le pouvoir formateur de la spontanéité en dehors du contexte traditionnel de l’éducation formelle. Ces moments, et notamment la façon dont ils perturbent l’ordre temporel ordinaire constituent des expériences vécues de notre vitalité. « Recourant à un langage clair et précis, In vivo captivera les philosophes et les intellectuels. Csepregi engage un dialogue entre diverses traditions, faisant appel aux meilleurs courants de plusieurs philosophies pour développer un point de vue unique ». - Gaëlle Fiasse, Université McGill. (shrink)

The recombinational repair of chromosomal double‐strand breaks (DSBs) is of critical importance to all organisms, who devote considerable genetic resources to ensuring such repair is accomplished. In Saccharomyces cerevisiae, DSB‐mediated recombination can be initiated synchronously by the conditional expression of two site‐specific endonucleases, HO or I‐Scel. DNA undergoing recombination can then be extracted at intervals and analyzed. Recombination initiated by meiotic‐specific DSBs can be followed in a similar fashion. This type of ‘in vivo biochemistry’ has been used to describe (...) several discrete steps in two different homologous recombination pathways: gene conversion and single‐strand annealing. The roles of specific proteins during recombination can be established by examining DNA in strains deleted for the corresponding gene. These same approaches are now becoming available for the study of recombination in both higher plants and animals. Physical monitoring can also be used to analyze nonhomologous recombination events, whose mechanisms appear to be conserved from yeast to mammals. (shrink)

Pagando por suas alforrias, batizando seus filhos, enterrando seus mortos. Além do trabalhado pesado ainda havia espaço para afetividade, vida sexual, desejos e vontades das mulheres que viviam no sul da capitania. E não faltavam acusações de sodomias, feitiçarias, seduções, bigamias. Este texto é fruto de um projeto de pesquisa em andamento que perscruta mulheres africanas no sul da Bahia e desdobra-se em duas partes de suas vidas no período colonial e imperial, tomando-as não só como objetos de pesquisa, mas (...) dando-lhes a condição de serem narradas como protagonistas apoiadas em vastas e inéditas fontes. Este artigo optou por trazê-las no período colonial. Quanto ao período aludido sobressaiu-se nas fontes dois aspectos: as pecadoras denunciadas estavam relacionadas ou às práticas de feitiçaria ou à vida sexual e afetiva destoante do que preconizava as leis cristãs, sobretudo nos Cadernos do Promotor. As fontes utilizadas foram os Cadernos do Promotor, as Correspondências trocadas entre autoridades e uma memória descritiva sobre ser mulher, ser africana e ser escravizada ou forra em diferentes discursos. Palavras-chave: Africanas, Cadernos do Promotor, Pecados. (shrink)

The course of human life, punctuated by unexpected and transformative moments, is never uniform. What are the characteristics of such life-defining moments, what responses do they evoke, and how do they transform the lives of those who experience them? In Vivo explores foundational questions and pivotal moments of the human experience – engagement with a foreign culture, the decision to break free from unfortunate experiences, a generous action undertaken in the context of an otherwise regular day – in terms (...) of their life-altering potential. Through illustrative examples, both real and fictional, Csepregi reveals the primacy of personal feelings in shaping human life and demonstrates the formative power of spontaneity outside the traditional context of formal education. These moments, and particularly the way they disrupt ordinary temporal order, Csepregi argues, are the lived experiences of our vitality. In an age marked by increasing anxiety about the homogenizing tendencies of contemporary life, In Vivo is timely and revelatory. Informed by a range of philosophical thinking and examples from art, music, and literature, it illustrates opportunities for meaningful reflection that are available to everyone, and urges the reader to engage with them. (shrink)

Resumo: A epistemologia é usualmente neutra quanto aos diferentes tipos de objeto que a ciência investiga. Entretanto, seguindo Popper e Stengers, podemos descrever que os objetos vivos impõem à investigação científica uma mudança radical em sua abordagem. As ciências que lidam com objetos vivos não podem mais compartilhar os pressupostos do experimentalismo: o ser vivo não é isolável de seu ambiente. Palavras-chave: Conhecimento; Evolução; Filosofia da ciência.

Cranial electrical stimulation has been applied at various current levels in both adults and children with neurological conditions with seemingly promising but somewhat inconsistent results. Stimulation-induced spatial electric fields within a specific brain region are likely a significant contributing factor for the biological effects. Although several simulation models have been used to predict EF distributions in the brain, these models actually have not been validated by in vivo CES-induced EF measurements in the live human brain. This study directly measured (...) the CES-induced voltage changes with implanted stereotactic-electroencephalographic electrodes in twenty-one epilepsy participants and then compared these measured values with the simulated ones obtained from the personalized models. In addition, we further investigated the influence of stimulation frequency, intensity, electrode montage and age on EFs in parts of participants. We found both measured voltages and EFs obtained in vivo are highly correlated with the predicted ones in our cohort. In white matter and gray matter, the measured voltages linearly increased when the stimulation intensity increased from 5 to 500 μA but showed no significant changes with changing stimulation frequency from 0.5 to 200 Hz. Electrode montage, but not age, significantly affects the distribution of the EFs. Our in vivo measurements demonstrate that the individualized simulation model can reliably predict the CES-induced EFs in both adults and children. It also confirms that the CES-induced EFs highly depend on the electrode montages and individual anatomical features. (shrink)

manganese (Mn2+) enhanced MRI (MEMRI) to study neuronal connectivity in vivo opens the possibility to these studies. However, several drawbacks exist that challenge its applicability. High Mn2+ concentrations produce cytotoxic effects that can perturb the circuits under study. In the other hand, the MR signal is..

En 1888, Nietzsche se presenta a la humanidad antes del gran asalto: La transvaloración de todos los valores. Se lamenta de no ser reconocido, de ser confundido, de no ser visto ni oído por otros. Por eso, decide autopresentarse a través de un libro que intenta mostrarlo como capaz de realizar y soportar la propia transvaloración. El resultado: un libro peculiar e incomprensible, para muchos la obra de un delirante. Pero es también, para nosotros, la obra que muestra a un (...) Nietzsche intentando la inmortalidad a través de su escritura. (shrink)

El trabajo vivo no es una categoría económica, sino que forma parte de la actividad práctica humana que surge del fundamento mismo de la vida. Sin embargo, en el sistema de producción capitalista el trabajo vivo es convertido en la determinación esencial del capital en su proceso de valorización. Así, mientras lo activo y lo subjetivo queda transformado en una objetividad muerta para los propios productores, el capital llega a su plena perfección con ello: el plusvalor. Partiendo de (...) un análisis ontológico de la actividad laboral encontramos que el trabajo vivo es la categoría simple sobre la que se articula el resto de categorías económicas. La perspectiva filosófica ha de permitirnos analizar, mostrar y especificar las íntimas relaciones que se establecen entre el capital y el trabajo, así como las metamorfosis y transformaciones que ha de sufrir el trabajo vivo para convertirse en entelequia del capital, es decir, el tránsito de lo vivo a lo muerto. (shrink)

A controversial hypothesis in RNA biology is that extracellular microRNAs (miRNAs), including those in biofluids, have non‐cell‐autonomous activities. Several studies have characterized biofluid miRNA profiles in healthy or diseased individuals but generally have failed to identify distinct disease signatures. It remains unclear whether alterations in fluid miRNA levels are simply indicators of physiological change or whether miRNAs are taken up by new cells at concentrations sufficient to affect gene expression. There are limitations to biofluid miRNA studies performed to date: methodology (...) for isolating and quantifying biofluid miRNAs is not standardized across studies; mechanistic details of miRNA release and uptake are incomplete; and efforts to assess non‐cell‐autonomous effects of extracellular miRNAs have employed predominantly in vitro strategies. We describe controversies and questions that need to be addressed to test possible in vivo roles of extracellular miRNAs and propose model organisms with rich genetic toolkits for carrying out in vivo functional analyses. (shrink)

Hippocampal neurogenesis has been implicated in the pathogenesis of and recovery from depression. However, most of the underlying studies were endpoint investigations in experimental animals yielding conflicting results, and it has been under debate to which extent these results could be transferred to human patients. Now, researchers have developed a powerful new tool to address these questions by a non‐invasive method in humans and animals in vivo, using magnetic resonance spectroscopy to detect a biomarker for proliferating progenitor cells that (...) give rise to new neurons.1 This makes it possible to study the role of neural progenitor cells in a wide variety of human brain disorders. BioEssays 30:806–810, 2008. © 2008 Wiley Periodicals, Inc. (shrink)

La frase sobre el logos escrito como “reflejo” del discurso vivo y animado del que sabe (cf. Fedro, 276a8-9) hay que referirla al diálogo platónico: “el que sabe” es el dialéctico, que gracias a los conocimientos a los que ha accedido por medio de la teoría de las Ideas, “sabe cómo es la verdad” (ibid., 278c4-5). Tres capacidades tiene, según Platón, solo el discurso vivo del “que sabe”: puede responder las preguntas que se le planteen, puede buscar el (...) receptor adecuado o quedar en silencio ante aquellos que no tienen que ver con filosofía y, en caso de un ataque, puede acudir en ayuda de su argumento con cuestiones que pertenecen a un nivel superior (cf. ibid., 275d4-276a9, 278c4-e3). El análisis de estas expresiones de Platón en su contexto muestra que la interpretación usual que, desde hace casi 200 años, le atribuye estas capacidades de “el que sabe” al diálogo escrito, no hace justicia al texto de la crítica de la escritura y va en contra de su sentido. Esta constatación exige una orientación radicalmente nueva de la hermenéutica de los diálogos platónicos. Hay que reconocer que Platón concibió sus diálogos (cada uno en particular y todos en conjunto) como unidades literarias y filosóficas que no tienen un carácter autárquico. Los diálogos apuntan consistentemente más allá de ellos mismos hacia la doctrina oral de Platón. --- “Reflection of the living discourse. ¿What is a Platonic dialogue and what does it pretend?”. Plato’s word in the Phaedrus about the written logos as an “image” of the living logos of the man who knows (cf. Phaedrus, 276a8-9) has to be referred to his own dialogues: the “man who knows” is the dialectician, who “knows the truth” (cf. ibid., 278c4-5) thanks to the insights he has gained by way of his use of the Theory of Ideas. There are three things, according to Plato, which can be achieved only by the living logos of “the man who knows”: his logoscan give answers to new questions, can choose the right sort of recipient and remain silent to those who have nothing to do with philosophy, and, in case it should be attacked, it can help its argument with “things of higher importance” (cf. ibid., 275d4-276a9, 278c4-e3). An analyse of these views and concepts of Plato in their context shows that the conventional interpretation, current since almost 200 years, according to which the written Platonic dialogue can do the same three things as the “man who knows”, is contrary to the wording of Plato’s “Criticism of Writing” and incompatible with its sense. This result leads to a new orientation regarding the hermeneutics of the Platonic dialogue. We have to acknowledge that Plato did not mean his dialogues (each one by itself, and their entirety as a whole) to be autarchic of self-sufficient literary entities. The dialogues point consistently beyond themselves to Plato’s oral teaching. (shrink)

Csepregi, Gabor In Vivo: A Phenomenology of Life-Defining MomentsMontreal: McGill-Queen’s University Press, 216 p.ISBN 978-0773556638.

Nanomaterials have the promise of revolutionizing current treatment and diagnosis of diseases, which has led to 33 nanotherapeutics drugs currently on the market and many more in various stages of clinical trials. With an increasing number of products available and in development, along with the unique, emergent properties of the nanoparticle therapeutics themselves, regulatory agencies are now faced with decisions regarding the regulation of such novel technologies. Regulatory guidance, particularly in pre-clinical stages, has the potential to facilitate quick and safe (...) development of these novel materials, but new regulation beyond what is currently in place must be justified in a clear and distinctive toxic response. Herein, we examine literature that compares and correlates in vivo and in vitro nanotoxicity studies to gain a deeper understanding of the modes of nanoparticle toxicity. Additionally, this comparison aims to identify clear and unique toxicity responses caused by nanoparticles, which informs our perspective on pre-clinical nanotherapeutic oversight. (shrink)

Much of the focus of the published 2011 symposium that inspired this work focused on the question, “When have you reduced risk enough to move from bench/animal studies to ‘first in-human’ studies?” Building applied research ethics related to nanotherapeutics requires bench and clinical scientists to have a clear vision about how to test nanotherapeutic safety, and it is clear that there is still much to be considered at the steps before “in-human” assessment. Herein, the perspective of the bench scientist is (...) brought to bear on using in vivo and in vitro models to assess the safety of nanotherapeutics. Much of this work falls under the purview of the field of nanotoxicology that aims to understand the toxicological impact of engineered nanoscale materials. Engineered nanomaterials include a wide variety of materials that are manipulated and controlled on the nanoscale level where, typically, the nanoparticle or nanomaterial has some dimension that is less than 100 nm. (shrink)