Large deletions and genomic re‐arrangements are increasingly recognized as common products of double‐strand break repair at Clustered Regularly Interspaced, Short Palindromic Repeats ‐ CRISPR associated protein 9 (CRISPR/Cas9) on‐target sites. Together with well‐known off‐target editing products from Cas9 target misrecognition, these are important limitations, that need to be addressed. Rigorous assessment of Cas9‐editing is necessary to ensure validity of observed phenotypes in Cas9‐edited cell‐lines and model organisms. Here the mechanisms of Cas9 specificity, and strategies to assess and mitigate unwanted effects (...) of Cas9 editing are reviewed; covering guide‐RNA design, RNA modifications, Cas9 modifications, control of Cas9 activity; computational prediction for off‐targets, and experimental methods for detecting Cas9 cleavage. Although recognition of the prevalence of on‐ and off‐target effects of Cas9 editing has increased in recent years, broader uptake across the gene editing community will be important in determining the specificity of Cas9 across diverse applications and organisms. (shrink)

The objective of the study was to develop a structural model that explores the relationship between Mathematics Performance and students’ self-regulated learning skills, grit, and expectancy-value towards science, technology, engineering and mathematics (STEM). The research collected survey data from 664 senior high school students from 17 STEM high schools, and conducted a covariance-based structural equation modeling (SEM) analysis. The results of the SEM analysis indicate that the Re-specified Self-Regulated Learning Skill – Expectancy-Value towards STEM – Grit – Mathematics Performance (Re-specified (...) SRL-EV-GR-MP) model is the most parsimonious fit, offering the best empirical support for the theoretical model of the study. The research findings suggest that the mathematics performance of senior high school students in STEM curriculum is attributed to their high expectancies for success and perceived values of the STEM tasks, high grit, and high self-regulated learning skills. Moreover, the research also observed evidence of mediating and moderating grit effects in the concurrent effects of expectancy-values towards STEM and self-regulated learning skills towards students’ mathematics performance. (shrink)

À partir de la reconstitution d’un contexte polémique divisant, dans le dernier quart du ve siècle, les tenants d’une conception traditionnelle de la religion et des savoirs d’une part, et les partisans d’une curiosité nouvelle pour l’étude des « choses d’en haut » (meteora) d’autre part, cet article se propose d’examiner la manière dont l’élément aérien a été investi par les seconds pour devenir le support matériel de l’« invisible » et se substituer ainsi à la divinité. L’analyse des caractéristiques (...) précises que certains philosophes et médecins ont associées à l’air permettra de mettre en évidence le rôle de puissance cosmologique et biologique qui a pu lui être reconnu dans cette tradition de pensée « aérienne ». (shrink)

Investigating the theoretical and empirical relationships between transparency and trust in the context of surveillance, this volume argues that neither transparency nor trust provides a simple and self-evident path for mitigating the negative political and social consequences of state surveillance practices. Dominant in both the scholarly literature and public debate is the conviction that transparency can promote better-informed decisions, greater oversight, and restore trust damaged by the secrecy of surveillance. The contributions to this volume challenge this conventional wisdom by considering (...) how relations of trust and policies of transparency are modulated by underlying power asymmetries, socio-historical legacies, economic structures, and institutional constraints. They study trust and transparency as embedded in specific socio-political contexts to show how, under certain conditions, transparency can become a tool of social control that erodes trust, while mistrust-rather than trust-can sometimes offer the most promising approach to safeguarding rights and freedom in an age of surveillance. The first book addressing the inter-relationship of trust, transparency, and surveillance practices, this volume will be of interest to scholars and students of surveillance studies as well as appeal to an interdisciplinary audience given the contributions come from political science, sociology, philosophy, law, and civil society. (shrink)

Max Weber's distinction in "Politics as a Vocation" between the ethic of conviction and the ethic of responsibility is best understood as a distinction between mutually exclusive ethical worldviews. Interpretations that correlate the two ethics with Weber's distinction between value-rational social action and instrumental-rational social action are misleading since Weber assumes that both types of rational social action are present in both ethics. The ethic of conviction recognizes a given hierarchy of values as the context for moral endeavor. The ethic (...) of responsibility acknowledges value obligations, but assumes the absence of any given hierarchy of values and the inevitability of value conflict as the context for moral endeavor. When interpreted in the context of his multilayered understanding of value conflict, Weber's ethic of responsibility emerges as a coherent ethical perspective. (shrink)

This manuscript describes the responses and correlates of outpatients with schizophrenia spectrum disorders to a tool designed to measure comprehension before obtaining informed consent for research participation. We used the Evaluation to Sign Consent form to document comprehension in 100 outpatients as part of their consent to participate in an ongoing study of an exercise intervention. The findings suggest that using this form is a feasible and acceptable approach to documenting comprehension of research procedures prior to obtaining informed consent. Age (...) 49 years and older and the receipt of intramuscular antipsychotic medication predicted the need for additional assistance to complete the Evaluation to Sign Consent form successfully (χ2 = 8.29, P = 0.016). Nurse researchers should consider documenting comprehension with this tool owing to its availability, time efficiency and utility. (shrink)

El texto presenta un estudio sobre Juán Amós Comenio (1592-1670) como clásico del pensamiento educativo, el hilo conductor ha consistido en tener presente el carácter sociocultural de la educación, es decir, en no perder de vista una verdad elemental: las aportaciones en el ámbito educativo, trátese de discursos, de prácticas, o bien de ambos, aún cuando pudieran presentarse ante nuestros ojos con cierto grado de abstracción, son elaboraciones que proceden de personas concretas que viven coordenadas de tiempo y espacio precisas (...) y que por ende, se vuelven comprensibles con mayor profundidad y plenitud en la medida que uno se aproxima a la forma en que vivió esta persona. Este libro constituye un proyecto de investigación más amplio, pero prioritariamente aborda aspectos de las atmósferas del siglo XVII propias de la Europa protestante que marcan la vida de Comenio b) algunas tradiciones y legados que sustentan su proyecto educativo. (shrink)

This paper aims to address the difficulties of high school students in bridging their computational understanding with their visualization skills in understanding the notion of the limits in their calculus class. This research used a pre-experimental one-group pretest-posttest design research on 62 grade 10 students enrolled in the Science, Technology, and Engineering Program (STEP) in one of the public high schools in Zamboanga del Sur, Philippines. A series of remedial sessions were given to help them understand the function values, one-sided (...) limits, limits of a function, and its discontinuities with the aid of the GeoGebra. Results revealed that there is a significant improvement in their mathematics performance before and after the remediation, t(61) = -19.99, p<.05. The real-time feedback provided by the drawing interface of GeoGebra has provided the students the insights into how objects behave in geometrical space, bridging their understanding in locating the values shown by the computational algebraic processes. Additionally, the significant difference in the achievement reveals a large effect size (Cohen’s =2.54) which could be accounted for by the remediation using interactive activities in the GeoGebra. (shrink)

La humildad copernicana establece que el Universo es un lugar homogéneo en el que las leyes aplican indistintamente en cualquier lugar, es decir, no existe un centro o lugar privilegiado. En ese sentido el lugar que ocupa la Tierra en el Universo es como cualquier otro, nada especial y mucho menos relacionado al favor de un Dios omnipotente cuya creación favorita sea el ser humano. Siendo esto así, sería entonces factible pensar la existencia de muchos otros planetas que como la (...) Tierra alberguen vida y sustenten civilizaciones tan o más avanzadas como la nuestra. Sin embargo, ¿por qué aún no hemos contactado con otras civilizaciones? Esto último se conoce como la paradoja de Fermi. ¿Por qué si la Tierra es solo un punto más en el Universo parece que no hay otras Tierras y otros seres inteligentes con quienes contactar? Una posible solución que intentamos discutir en el presente texto es la Hipótesis de la Tierra Rara, según la cual, en efecto, el planeta Tierra es una cosa extraordinaria en el universo. Más allá de discutir la plausibilidad de esta hipótesis frente a otras, interesa puntualizar algunos corolarios que se siguen de la misma, y su implicación para el copernicanismo desde un punto de vista filosófico. (shrink)

In this paper we defend that elective ventilation, even if conceived as the instrument to maximise the chances of organ recovery, is mainly the means to provide the patient who is dying with a dignified death in several ways, one of them being the possibility of becoming an organ donor. Because EV does not harm the patient and permits the medical team a better assessment of the patient's clinical trajectory and a better management of the dying process by the family, (...) EV does not violate the principle of non-beneficence nor the principle of autonomy if we restrict the initiation of EV to those cases in which it is not known what the previous wishes of the patient were as regards to his or her care at the end of life. (shrink)

Protecting the privacy of individually-identifiable health data and promoting the public’s health often seem at odds. Privacy advocates consistently seek to limit the acquisition, use, and disclosure of identifiable health information in governmental and private sector settings. Their concerns relate to misuses or wrongful disclosures of sensitive health data that can lead to discrimination and stigmatization against individuals. Public health practitioners, on the other hand, seek regular, ongoing access to and use of identifiable health information to accomplish important public health (...) objectives. The collection and use of identifiable health data by federal, tribal, state, and local health authorities support nearly all public health functions and goals.Identifiable health data are the lifeblood of public health practice. When aggregated, these data help authorities monitor the incidence, patterns, and trends of injury and disease in populations. Health data are acquired by public health authorities through testing, screening, and treatment programs. (shrink)

Current economic conditions have coincided with the implementation of the Health Insurance Portability and Accountability Act and forced public health officials to consider how to ethically incorporate compliance into their already strained budgets, while maintaining the integrity and intent of the legislation.As of April 14, 2003, the HIPAA Privacy Rule provides a new federal floor of protections for personal health information. The Privacy Rule establishes standards for the protection of health information held by many physicians’ offices, health plans, and health (...) care clearinghouses. The Rule protects personal health information by establishing conditions regulating the use and disclosure of individually identifiable health information by these entities, also referred to as covered entities. The Rule does not prevent the daily operations of health care establishments. (shrink)

This paper questions the conceptual and pragmatic worth of the category of relational values. Combining philosophical reasoning with ethnographic field-work in Wallmapu/Chile, I analyse a variety of ways in which people think about, value and behave toward the land. I thereby demonstrate that relational-ity is inherent to held, instrumental and intrinsic values. This means that there is no meaningful way in which to distinguish relational values from more familiar types of values. Yet, to be able to argue that a distinct (...) class of relational values exists, those who have proposed the term have been compelled to silence or downplay the relational natures of those other values. This has the perverse effect of confining, rather than promoting, relational thinking. (shrink)

The purpose of the paper is to investigate into the peculiarities of the reflection of sense as the main idea of modern European philosophy. This idea refers to self-consciousness, about which modern empirical philosophy speaks in the language of reflection (J. Locke), and rationalism - in the language of Cogito (R. Descartes). For Kant, Cogito is a transcendental condition for the formation of concepts, and therefore reflection should be transcendental. According to Husserl, the main task of phenomenological reflection was to (...) obtain essences of processes, to build things from the acts of Cogito. In Husserl's words, reflection "carries the world as a meaningful sense". Such a sense (smysl) becomes a problem escaping both from analysis and interpretation. Its peculiarity lies in the deontological status of sense: the phrase "a sense should be" connects the sense with a realm of duty, and duty provides a correlation between Ego and integrity of the world, language and culture. Reflection of sense (smysl) becomes the process of comprehension, the mechanism of formation of the whole set of meanings in the culture. (shrink)

In this paper we defend that elective ventilation (EV), even if conceived as the instrument to maximise the chances of organ recovery, is mainly the means to provide the patient who is dying with a dignified death in several ways, one of them being the possibility of becoming an organ donor. Because EV does not harm the patient and permits the medical team a better assessment of the patient's clinical trajectory and a better management of the dying process by the (...) family, EV does not violate the principle of non-beneficence nor the principle of autonomy if we restrict the initiation of EV to those cases in which it is not known what the previous wishes of the patient were as regards to his or her care at the end of life. (shrink)

Resumen: El artículo establece paralelos entre el Concilio Vaticano II (1962-1965) y la Conferencia General del Episcopado Latinoamericano (Medellín, 1968), bajo la perspectiva común de la historicidade del “mistério de la Iglesia”. Un paralelismo fundamental: la creación del CELAM (1955) anticipa en la práctica la doctrina de la colegialidad episcopal, promulgada por el Concilio ( Lumem Gentium 22). Bajo el foco de la colegialidade eclesial, toda la intensa actividad de preparación de la Conferencia de Medellín, así como su realización y (...) su documento final, se construye “a la luz del Concilio”. Desde la autocomprensión de la Iglesia inserida en la historia –expresada y compartida en los dos acontecimentos- se formulan los grandes temas y compromisos de la Iglesia latino-americana: la opción evangélica por los pobres; la nueva manera de ser Iglesia que se concreta en las comunidades eclesiales de base; y la dimensión teológica de las aspiraciones y exigências de liberación, formulada posteriormente por la teologia de la liberación. Leídos en clave teológica, el Concilio y Medellín fueron un signo de los tiempos, un kairós en el caminar histórico de la Iglesia hacia el Reino. La reflexiópn se refiere también a un actual “tiempo de exilio” de la Iglesia, que se caracteriza por la distancia de las grandes y exigentes convocaciones del Concilio y de Medellín. En el exilio, voces proféticas conclaman a superar el olvido de la historia. Palabras-clave : Iglesia. Concilio Vaticano II. CELAM. Conferencia de MedellíThis article draws parallels between the Second Vatican Council (1962 – 1965) and the Second General Conference of Latin American Bishops (Medellín, 1968), under the common view of the historicity of the “mystery of the Church”. A basic parallel arises: the creation of CELAM (1955) anticipates the doctrine of episcopal collegiality, promulgated by the Council (Lumen Gentium 22). Under the focus of the ecclesial collegiate, all the intensive activity of preparation for the Conference of Medellin, its realization and its final document, were made through the Council inspiration. By comprehending the Church as inserted in history the major themes and commitments of the Latin American Church are formulated: the evangelical option for the poor, the new way of being church founded in ecclesial communities, the theological dimensions of the aspirations and demands for liberation later formulated in Liberation Theology. Seen with theological eyes, the Council and the Conference of Medellín were a sign of the times, a historical movement of the Church towards the Kingdom of God. This discussion also refers to the current time of the Church as a “time in exile”. The present time is characterized by its detachment from the demands of the Council and of Medellín. In exile, prophetic voices call on to overcome the forgetting of history. Keywords: Church. Vatican Council II. CELAM. Conference of Medellín. - DOI: 10.5752/P.2175-5841.2011v9n24p1233. (shrink)

La presente investigación aborda los distintos modelos urbanísticos que propuso Le Corbusier a lo largo de toda su trayectoria. Estos modelos principales, cuantificándose hasta cuatro, además de otros de menor entidad, muestran la evolución en la conceptualización y las características que propuso para la ciudad del siglo XX. Su aplicación a las diferentes ciudades, tanto interiores como litorales, evidencian tanto la diversidad de su urbanismo como la adaptabilidad de los mencionados modelos a casos reales. De este modo, se procura superar (...) las visiones parciales que habitualmente se hacen sobre toda su obra urbana. (shrink)

This article discusses the specifics of social and philosophic approach to studying society in the context of two basic tendencies in modern social researches: interdisciplinary studies and commitment to search of meaning/sense. Both tendencies point to a special subject of this research – text. The authors believe that social philosophy has a particular characteristic that should be preserved and an introduction of the concept “public text” will help the preservation. Publicity of social text is determined as its special status in (...) the society consisting in creation of socially meaningful senses. Among public texts the authors single out publicistic texts that are of special format and possess expressed socially transforming potential. This article proposes valuative model as a method of analytically clear and reflexive reading of such text. It is being established that sociocultural research of modern days will have broader perspective in regards to specificity and thingness if it is involved with studying (analysis and interpretation) of namely texts. Social and philosophic research itself becomes text as well – mainly author’s and intentionally published, that preserves special features of philosophical text in general. The concept of “social text” loses virtual status at that, philologism as a regular consequence of modern interdisciplinary studies is being overcome and the subject of social and philosophical reflection is obtained from the area of text creation. The authors note two main research options of social philosophy – generalization of work results from experts in cultural studies, social studies, political studies, etc. (critical and methodological); determination and comprehension of socially important intents (reflexive and textological). (shrink)

Hymenoplasty is a surgical procedure requested by women who are expected to remain virgins until marriage. In this article, I assess the ethical and legal challenges raised by this request, both for the individual physician and for the health care system. I argue that performing hymenoplasty is not always an unethical practice and that, under certain conditions, it should be provided by the health care system.

One of the most controversial aspects in uncontrolled donation of organs after circulatory death is the initiation of preservation measures before death. I argue that in so-called opting-out systems only under very stringent conditions we might presume consent to the instauration of those measures. Given its current legal framework, I claim that this is not the case of Spain, a well-known country in which consent is presumed—albeit only formally—and where uDCD is currently practiced.

This article introduces a new scale to measure executive servant leadership, situating the need for this scale within the context of ethical leadership and its impacts on followers, organizations and the greater society. The literature on servant leadership is reviewed and servant leadership is compared to other concepts that share dimensions of ethical leadership (e.g., transformational, authentic, and spiritual leadership). Next, the Executive Servant Leadership Scale (ESLS) is introduced, and its contributions and limitations discussed. We conclude with an agenda for (...) future research, describing ways the measure can be used to test hypotheses about organizational moral climate, ethical organizational culture, corporate responsibility, and institutional theory. (shrink)

In this paper, we provide a new framework for understanding infant-feeding-related maternal guilt and shame, placing these in the context of feminist theoretical and psychological accounts of the emotions of self-assessment. Whereas breastfeeding advocacy has been critiqued for its perceived role in inducing maternal guilt, we argue that the emotion women often feel surrounding infant feeding may be better conceptualized as shame in its tendency to involve a negative self-assessment—a failure to achieve an idealized notion of good motherhood. Further, we (...) suggest, both formula-feeding and breastfeeding mothers experience shame: the former report feeling that they fail to live up to ideals of womanhood and motherhood, and the latter transgress cultural expectations regarding feminine modesty. The problem, then, is the degree to which mothers are vulnerable to shame generally, regardless of infant feeding practices. As an emotion that is less adaptive and potentially more damaging than guilt, shame ought to be the focus of resistance for both feminists and breastfeeding advocates, who need to work in conjunction with women to oppose this shame by assisting them in constructing their own ideals of good motherhood that incorporate a sense of self-concern. (shrink)

Hymenoplasty is a minor surgical procedure requested by women who, for cultural or religious reasons, need to remain a virgin until marriage. In this article I assess whether the public healthcare system of a liberal state should provide it as part of a policy of multicultural accommodation. I conclude that, in order to remain loyal to certain ethical ideals linked to the rule of law, liberal states should give access to hymenoplasty only to women for whom premarital virginity is a (...) precondition for their flourishing within their community and who say they are victims of sexual assault. (shrink)

The so-called uncontrolled donation after circulatory determination of death (uDCDD) have been implemented in several countries, including Spain and France, to increase the availability of organs for transplantation. These protocols allow obtaining kidneys, livers and lungs of patients who do not survive cardio-pulmonary resuscitation performed in out-of-hospital settings. Simultaneously with the development and recent proliferation of these protocols, some emergency teams have begun to employ unconventional methods of CPR, with still uncertain but promising results. The coexistence of these two possibilities (...) raises some questions: At what point should attempts to save a patient with circulatory arrest be abandoned so that this individual can begin to be considered as a potential organ donor? How to manage potential conflicts of loyalties that may arise within emergency and procurement teams? How should the information to the families of those patients and potential organ donors be managed? This article gathers the conclusions of a workshop in which an interdisciplinary working group addressed these and other questions related to DCDD and non-conventional CPR. (shrink)