Enzyme engineering allows to explore sequence diversity in search for new properties. The scientific literature is populated with methods to create enzyme libraries for engineering purposes, however, choosing a suitable method for the creation of mutant libraries can be daunting, in particular for the novices. Here, we address both novices and experts: how can one enter the arena of enzyme library design and what guidelines can advanced users apply to select strategies best suited to their purpose? Section I is dedicated (...) to the novices and presents an overview of established and standard methods for library creation, as well as available commercial solutions. The expert will discover an up‐to‐date tool to freshen up their repertoire (Section I) and learn of the newest methods that are likely to become a mainstay (Section II). We focus primarily on in vitro methods, presenting the advantages of each method. Our ultimate aim is to offer a selection of methods/strategies that we believe to be most useful to the enzyme engineer, whether a first‐timer or a seasoned user. (shrink)

Despite their diversity and ecological importance, many areas of the SAR—Stramenopila, Alveolata, and Rhizaria—clade are poorly understood as the majority of SAR species lack molecular data and only 5% of species are from well-sampled families. Here, we review and summarize the state of knowledge about the three major clades of SAR, describing the diversity within each clade and identifying synapomorphies when possible. We also assess the “dark area” of SAR: the morphologically described species that are missing molecular data. The majority (...) of molecular data for SAR lineages are characterized from marine samples and vertebrate hosts, highlighting the need for additional research effort in areas such as freshwater and terrestrial habitats and “non-vertebrate” hosts. We also describe the paucity of data on the biogeography of SAR species, and point to opportunities to illuminate diversity in this major eukaryotic clade. See also the video abstract here: https://youtu.be/_VUXqaX19Rw. Despite their diversity, abundance, and importance, fewer than 10% of the species within the SAR—Stramenopila, Alveolata, and Rhizaria—clade have been assessed using molecular tools. Only a small percentage of the molecular records contain information on ecology or have associate location data, indicating a tremendous dark area. (shrink)

Society is the ensemble/the whole of the relations with the others, it is their form based on the natural needs : the perpetuation of the species, the playful expression, the language, the thinking, the communication, the inter-subjectivity report. In the evolution of psychology of human development, sport contributes to the improvement of the body in relation to the environment; of the cognitive, moral development of language, that of complex skills, sensory integrations, games with body schematics, which mobilize self-awareness and structure (...) through learning and experience. Thus, social and communication behaviours generate a mutually advantageous social repertoire. (shrink)

Preterm discharge effects: Relationship between the maternal experience and newborn's psychobiological regulation The purpose of this study is to investigate how the past experiences of mothers and their potentially traumatic events during pregnancy may have influenced the processes of psychobiological self-regulation and cognitive development in a child born preterm. Eighty children who had a gestational age of < 32 weeks were examined at the 9th month of the corrected age. The mothers and children were divided in two groups: multipara mothers (...) and their children and the primipara mothers and their children. These children were examined on the basis of sleep disorders, nutritional problems and mental development. Perinatal data from clinical observations and data from standardized developmental tests were used to describe the differences. The children of primipara mothers who had unexpected preterm delivery presented major incidences of sleep disorders while the children of primipara mothers, who programmed preterm delivery, presented a major incidences of nutrition problems. The predictability of preterm delivery has a major influence on the capacity of newborns for self-regulation in the case of primipara mothers. (shrink)

El presente artículo aborda la estrategia escritural de Daniela Catrileo. Se propone revisar la elaboración del concepto de piñen examinando la relación entre lenguaje y colonialidad, para, de ese modo, subrayar el potencial político y reivindicativo que comporta en la novela del mismo nombre, Piñen (2019). Dicha hipótesis articulará el ejercicio de lectura aquí propuesto, atendiendo principalmente las estrategias narrativas empleadas –servirse de un polilingüismo que rompe estructuras gramaticales– para descentrar el lenguaje de su determinismo colonial. Así, interpretamos dicha (...) estrategia escritural en consonancia con las potencias micropolíticas y descolonizadoras del arte descritas por Suely Rolnik y, en consecuencia, apostando por la posibilidad de diagramar un devenir minoritario que dispute los imaginarios del inconsciente colonial-capital. (shrink)

Over the last decade, research in semantics and pragmatics has started to increasingly incorporate new experimental methods from cognitive psychology. That this empirical stance on utterance interpretation has now reached maturity is revealed by two unmistakable symptoms: an increased reflection on the contextual methods used to elicit experimental data, and a continuous expansion of the linguistic phenomena and themes being investigated through these methods. The articles gathered in this volume testify to this very recent evolution of the field: a first (...) set of papers questions the interpretation or interpretability of data collected in non-naturalistic settings, while a second set reflects the extension of experimental methods to new topics. The volume offers an up-to-date overview of contemporary issues arising within the thriving field of Empirical Pragmatics. (shrink)

This article has as its theme the relationship between "power", "reason" and "religion" in O alienista, one of the central texts of Machado de Assis, written in 1881 and initially published in eleven chapters, between October 1881 and March 1882. It is intended to identify and analyze the power relations between the State ("Throne") and the Catholic Church ("Altar"); as well as to highlight the relations between "reason" (science) and its "absence" (madness); without neglecting the Machadian denunciations and criticisms of (...) scientism prevailing in the nineteenth century. To do so, at first, it is presented, even if summarily, the Author and the Tale; in a second moment, shedding lights on its "space", "time" and "action" its plot, necessary assumption for the last moment, where the "Power", "Reason" and "Religion" will be analyzed. This research is justified by the relevance of the author, the place that the alienist occupies in the set of his works, as well as the social relevance of his ironic criticisms. In this analysis, a bibliographic method of critical-analytical reading of the Tale was used, using also the interpretations of some scholars, listed in the References. (shrink)

For families all over the world, going through a pandemic has presented a number of challenges. In particular, social distancing measures involving the closure of schools and day care centers, as well as increasing work hours at home, made parents face very demanding situations. However, we know little about whether parents’ burnout levels are influenced by the age of their children. This study sought to determine whether levels of parental burnout are higher in families with at least one child under (...) the age of four than in families with older children. The second goal was to explore whether having children under 4 years of age moderates the relationship between parental cooperation and PB. A cross-sectional study was conducted with a sample of 651 participants since May 18th until August 27th, 2020. The main results showed that child age is a predictor of PB. Besides, having a child aged 0–4 years old moderates the relationship between parental cooperation and PB. Finally, it was found that in cases where there was at least one child under 4 years of age in the family, with one of the partners who worked remotely, the respondent’s PB rose by 7.9 points. The implications of these results with respect to the consideration of children’s ages in the different parental scenarios were discussed. (shrink)

In this event-related potential study a masking technique that prevents conscious perception of words and non-words through attentional distraction was used to reveal the temporal dynamics of word processing under non-conscious and conscious conditions. In the non-conscious condition, ERP responses differed between masked words and non-words from 112 to 160 ms after stimulus-onset over posterior brain areas. The early onset of the word–non-word differences was compatible with previous studies that reported non-conscious access to orthographic information within this time period. Moreover, (...) source localisations provided evidence for automatic activation of prelexical phonological information, whereas no evidence for non-conscious semantic processing was found. When subjects were informed about the masking technique, lexical differences occurred at later time intervals, suggesting conscious access to additional word related information. These results indicate that early visual word processing does not depend entirely on attentional resources, but that non-conscious processing probably is restricted to rather lower-level linguistic information. (shrink)

Leading philosophical models of curiosity represent it as a desiderative attitude whose content is a question, and which is satisfied by knowledge of the answer to that question. I argue that these models do not capture the distinctive character of a form of curiosity that I call 'erotic curiosity'. Erotic curiosity addresses itself not to a question but to an object whose significance for the inquirer is affective as well as epistemic. This form of curiosity is best understood by analogy (...) to erotic love as theorized by Plato in the Symposium. (shrink)

I-Language introduces the uninitiated to linguistics as cognitive science. In an engaging, down-to-earth style Daniela Isac and Charles Reiss give a crystal-clear demonstration of the application of the scientific method in linguistic theory. Their presentation of the research programme inspired and led by Noam Chomsky shows how the focus of theory and research in linguistics shifted from treating language as a disembodied, human-external entity to cognitive biolinguistics - the study of language as a human cognitive system embedded within the (...) mind/brain of each individual. The recurring theme of equivalence classes in linguistic computation ties together the presentation of material from phonology, morphology, syntax, and semantics. The same theme is used to help students understand the place of linguistics in the broader context of the cognitive sciences, by drawing on examples from vision, audition, and even animal cognition.This textbook is unique in its integration of empirical issues of linguistic analysis, engagement with philosophical questions that arise in the study of language, and treatment of the history of the field. Topics ranging from allophony to reduplication, ergativity, and negative polarity are invoked to show the implications of findings in cognitive biolinguistics for philosophical issues like reference, the mind-body problem, and nature-nurture debates.This textbook contains numerous exercises and guides for further reading as well as ideas for student projects. A companion website with guidance for instructors and answers to the exercises features a series of pdf slide presentations to accompany the teaching of each topic. (shrink)

It is generally believed that one argument advanced by Aristotle in favor of the political authority of the multitude is that large groups can make better decisions by pooling their knowledge than individuals or small groups can make alone. This is supported by two analogies, one apparently involving a “potluck dinner” and the other aesthetic judgment. This article suggests that that interpretation of Aristotle’s argument is implausible given the historical context and several features of the text. It argues that Aristotle’s (...) support for the rule of the multitude rested not on its superior knowledge but rather on his belief that the virtue of individuals can be aggregated and even amplified when they act collectively. This significantly alters our understanding of Aristotle’s political thought and presents a powerful alternative to the epistemic defenses of mass political activity popular today. (shrink)

Internationally, there is considerable inconsistency in the recognition and regulation of children's genetic connections outside the family. In the context of gamete and embryo donation, challenges for regulation seem endless. In this paper, I review some of the paths that have been taken to manage children' being closely genetically related to people outside their families. I do so against the background of recognising the importance of children's interests as moral status holders. I look at recent qualitative research involving donor-conceived people (...) and borrow their own words to make sense of a purported interest to know (of) their close genetic ties. I also review ways in which gamete donation may have facilitated new kinds of kinship, which are at the same time genetic and chosen. In short, in this paper, I explore what meaning there could be in genetic connections that is not about parenthood. Further, I argue that the focus on parenthood in previous work in this area may be detrimental to appreciating some of the goods that can be derived from close genetic connections. (shrink)

Self-referential sentences have troubled our understanding of language for centuries. The most famous self-referential sentence is probably the Liar, a sentence that says of itself that it is false. The Liar Paradox has encouraged many philosophers to establish theories of truth that manage to give a proper account of the truth predicate in a formal language. Kripke’s Fixed Point Theory from 1975 is one famous example of such a formal theory of truth that aims at giving a plausible notion of (...) truth by allowing truth value gaps. However, not only the concept of truth gives rise to paradoxes. A syntactical treatment of epistemic notions like belief and knowledge leads to contradictions that very much resemble the Liar Paradox. Therefore, it seems to be fruitful to apply the established theories of truth to epistemic concepts. In this paper, I will present one such attempt of solving the epistemic paradoxes: I adapt Kripke’s Fixed Point Theory and interpret truth, knowledge and belief within the framework of a partial logic. Thereby I find not only the fixed point of truth but also the fixed points of knowledge and belief. In this fixed point, the predicates of truth, belief and knowledge find their definite interpretation and the paradoxes are avoided. (shrink)

This article analyzes the autofigurative strategies of La travesía by Luisa Valenzuela in terms of the narratives of the self, the Argentine dictatorship and feminism. A correspondence is found between the disavowal of the voice, the structure of the autobiographical account and the portrait of the experience of the violence of the military regime and of gender. Initially, the novel is situated at the height of the narratives of the self that, with literary resources that are separated from the most (...) conservative autobiographical writings, denounce the impossibility of understanding the subject as univocal, while founding different approaches to the retelling of the experience. This location allows exploring the formal experiments that Valenzuela carries out around the autobiographical exercise and that highlight the crisis of the construction of memory and the exposure of contemporary subjectivity. In addition, it is shown how such strategies serve as a vehicle for reflection on feminism and dictatorship that permeate all of her work. For this, Valenzuela's notion of feminine writing is used, which puts the multiplicity of perspectives before the univocity of the autobiographical voice, the ambiguous before the precise, and the collective before the authoritarian. La travesía is, then, read as part of the works that come to the narration of the experience itself in a framework of the retelling of collective memory, with narrative games that point out the deictic futility of the self and the difficulty of recounting past time, without, therefore, abandoning the call for its reconstruction as a guarantee of non-repetition. (shrink)

O objetivo deste estudo foi analisar o bem-estar dos trabalhadores da saúde de um centro de reabilitação e readaptação, relacionando-o com a crença no mundo justo e com o lócus de controle. Participaram 146 profissionais que responderam a um questionário formado por perguntas sobre dados sócio-demog..

It is widely believed that we ought not to criticize others for wrongs that we ourselves have committed. The author draws out and challenges some of the background assumptions about the practice of criticism that underlie our attraction to this claim, such as the tendency to think of criticism either as a social sanction or as a didactic intervention. The author goes on to offer a taxonomy of cases in which the moral legitimacy of criticism is challenged on the grounds (...) that the critic him- or herself engages in the behavior that he or she criticizes in others. The author argues that, in each type of case, the would-be critics should not constrain their participation in moral discourse on the grounds that they are not themselves innocent of the wrongdoing they criticize in others. (shrink)

Crowdfunding is regarded a financing mechanism that could improve the funding opportunities of businesses with a pro-social orientation. Indeed, it is assumed that on digital platforms, citizens are inclined to provide more support to projects with a social benefit than to those without such an orientation, with significant ethical implications for the common good. Yet, extant empirical evidence regarding such a claim is still inconclusive. To advance this discussion, the present paper analyzes the conditions that influence crowd support for projects (...) displaying a pro-social orientation on a reward-based crowdfunding platform. To build our hypotheses, we adopt the lens of framing theory, and we relate it to the digital context. Beginning from the premise that, on crowdfunding platforms, information about projects has a hierarchical structure, we argue that a project’s success crucially depends on how much its proponent emphasizes the pro-social cues within this structure. Moreover, we propose that because pro-social cues demarcate a project over others, the effectiveness of pro-social framing is enhanced when the number of projects on the platform, i.e., its crowdedness, increases. Logit estimates on 8631 Kickstarter projects indicate that pro-social framing is positively associated with success as we expected, yet only when it is moderately emphasized. Further, we find that crowdedness on the platform positively moderates the effect of pro-social orientation on success. (shrink)