We have synthesized a 582,970-base pair Mycoplasma genitalium genome. This synthetic genome, named M. genitalium JCVI-1.0, contains all the genes of wild-type M. genitalium G37 except MG408, which was disrupted by an antibiotic marker to block pathogenicity and to allow for selection. To identify the genome as synthetic, we inserted "watermarks" at intergenic sites known to tolerate transposon insertions. Overlapping "cassettes" of 5 to 7 kilobases (kb), assembled from chemically synthesized oligonucleotides, were joined by in vitro recombination to produce intermediate (...) assemblies of approximately 24 kb, 72 kb ("1/8 genome"), and 144 kb ("1/4 genome"), which were all cloned as bacterial artificial chromosomes in Escherichia coli. Most of these intermediate clones were sequenced, and clones of all four 1/4 genomes with the correct sequence were identified. The complete synthetic genome was assembled by transformation-associated recombination cloning in the yeast Saccharomyces cerevisiae, then isolated and sequenced. A clone with the correct sequence was identified. The methods described here will be generally useful for constructing large DNA molecules from chemically synthesized pieces and also from combinations of natural and synthetic DNA segments. 10.1126/science.1151721. (shrink)

In the mid-nineteenth century, Karl Marx issued several critiques of political economy writings stressing the exclusive duality of states and the national economies. He argued that capitalism had characteristic features quite apart from those shaped by the idiosyncrasies of national economies. In the first part of this article, we critique the contemporary state-centered explanations for the industrialization of East Asia on same grounds. We claim that most political economists misinterpret or entirely ignore the significance of export-led industrialization, which is a (...) characteristic feature of East Asian capitalism. In the second part of the article, we demonstrate the importance of the retail revolution in the US and Europe on Asian industrialization. In particular, we show that the development of the sequential ordering system that is an inherent feature of Western-based contract manufacturing differentially shaped the industrial organization of East Asian economies. The resulting path-dependent trajectories of development, in turn, encouraged government policy-makers to ‘buy into’ trends of global capitalism in different ways. The trajectories also led business people to privilege and adapt some social institutions and cultural patterns over other ones. (shrink)

In recent years, with both the development of next‐generation sequencing approaches and the Supreme Court decision invalidating gene patents, declining costs have contributed to the emergence of a new model of hereditary cancer genetic testing. Multigene panel testing (or multiplex testing) involves using next‐generation sequencing technology to determine the sequence of multiple cancer‐susceptibility genes. In addition to high‐penetrance cancer‐susceptibility genes, multigene panels frequently include genes that are less robustly associated with cancer predisposition. Scientific understanding about associations between many specific moderate‐penetrance (...) gene variants and cancer risks is incomplete. The emergence of multigene panel tests has created unique challenges that may have meaningful psychosocial implications. Contrasted with the serial testing process, wherein patients consider the personal and clinical implications of each evaluated gene, with multigene panel testing, patients provide broad consent to whichever genes are included in a particular panel and then, after the test, receive in‐depth genetic counseling to clarify the distinct implications of their specific results. Consequently, patients undergoing multigene panel testing may have a less nuanced understanding of the test and its implications, and they may have fewer opportunities to self‐select against the receipt of particular types of genetic‐risk information. Evidence is conflicting regarding the emotional effects of this testing. (shrink)

Max Weber famously wrote that world images determine the tracks along which societies move. This article examines the relation between world images and the so-called tracks of society, which in this metaphor resemble the current concept of institution. This concept is now associated with the ‘new institutionalisms’ in social science and has no connection to the civilizational level of analysis that Weber used. This article reexamines Weber’s usage of the terms and employs the terms to analyze differences in the principles (...) of legitimacy between those emerging in the West and those in China, differences that Weber himself did not recognize. (shrink)

Governments must keep agricultural systems free of pests that threaten agricultural production and international trade. Biosecurity surveillance already makes use of a wide range of technologies, such as insect traps and lures, geographic information systems, and diagnostic biochemical tests. The rise of cheap and usable surveillance technologies such as remotely piloted aircraft systems presents value conflicts not addressed in international biosurveillance guidelines. The costs of keeping agriculture pest-free include privacy violations and reduced autonomy for farmers. We argue that physical and (...) digital privacy in the age of ubiquitous aerial and ground surveillance is a natural right to allow people to function freely on their land. Surveillance methods must be co-created and justified through using ethically defensible processes such as discourse theory, value-centred design and responsible innovation to forge a cooperative social contract between diverse stakeholders. We propose an ethical framework for biosurveillance activities that balances the collective benefits for food security with individual privacy: establish the boundaries of a biosurveillance social contract; justify surveillance operations for the farmers, researchers, industry, the public and regulators; give decision makers a reasonable measure of control over their personal and agricultural data; and choose surveillance methodologies that give the appropriate information. The benefits of incorporating an ethical framework for responsible biosurveillance innovation include increased participation and accumulated trust over time. Long term trust and cooperation will support food security, producing higher quality data overall and mitigating against anticipated information gaps that may emerge due to disrespecting landholder rights. (shrink)

High school students are at risk for increased sedentary behavior due in part to a decrease in physical activity throughout adolescence and to required sedentary behavior during much of the school day. The purpose of the current study is to examine the impact of using activity workstations in a high school English class for struggling readers. Twenty high school students participated in the study. The participants completed a 16-week study where each participant used an activity workstation for 8 weeks and (...) a traditional desk for 8 weeks in a crossover design for a 40-min period during normal class. They responded to a series of subjective questions about reading and schoolwork at the beginning and end of each 8-week session and followed the READ 180 program designed to help struggling readers during the study. The results indicated that academic performance increased in both desk conditions during the study and from the beginning to the end of the study. In addition, there was a significant improvement in items in the subjective survey related to reading, motivation, and schoolwork in both desk conditions across the study. The current results suggest that using an activity workstation in the classroom did not negatively affect academic performance or students’ perceptions of working on academic assignments compared to the traditional desk condition. These results indicate that activity workstations could be implemented in classrooms to provide students with a non-sedentary option during the school day thus increasing physical activity in students. (shrink)

In lieu of an abstract, here is a brief excerpt of the content:Mild Cognitive Impairment:Which Kind Is It?Andy Hamilton (bio)Keywordshuman kinds, mild cognitive impairment, multiple personality disorder, practical kinds, social constructionThere is much stimulating material in the Graham and Ritchie's paper (2006), concerning not just disease-classification but also the ethics of diagnosis. My concern is with the way in which they adduce Ian Hacking's views in the philosophy of science in support of their own. The authors quote with approval (...) his "dynamic nominalism" and its influence on Zachar's concept of "practical kinds." Hacking's view, as the authors quote it, is that "People classified in a certain way tend to conform to or grow into the ways that they are described; but they also evolve in their own ways, so that the classifications and descriptions have to be constantly revised" (1995, 21). The act of classifying changes both the classifier and the subject of classification, resulting in facts and categories that are both socially created and real. Hacking calls this "the looping effect of human kinds," and comments that "Multiple personality [disorder] is an almost too perfect illustration of this feedback effect" (1995, 21). The authors comment that Zachar (2000) builds on Hacking's position, discarding essentialist notions of classification that are limited to inherent biological properties, and turning instead to "practical kinds—nonarbitrary cultural artifacts intended to meet pragmatic needs (e.g. therapeutic, predictive prognostic, labeling effects, and physiology)" (Graham and Ritchie 2006, 36). Practical kinds, they believe, offer a way out of the clinical approach focused on mild cognitive impairment (MCI) as a precursor to, or mild form of, Alzheimer's disease. The inclination of the authors, as I read them, is to treat MCI as a practical or human kind, and not, like Alzheimer's disease, as a natural kind.My concern is whether the "looping effect of human kinds" can be transferred to the case of MCI from Hacking's original case study in Rewriting the Soul, multiple personality disorder (MPD). Hacking wished there to undermine the traditional debate over whether MPD is a "social or psychiatric artifact." He claims, correctly I think, that the presupposition behind the question "Is MPD a real disorder as opposed to a product of social circumstances?" should be rejected. The fact that a disorder is a product of social circumstances does not make it less real (1995, 12). On the looping of human kinds, Hacking comments that "The concept of child abuse [implicated in MPD] may itself be so made and molded by attempts at knowledge and intervention, and social reaction to these studies, that there is no stable object, child abuse, to have knowledge about." This is a "no fact of the matter" response, and it [End Page 51] is based on the recognition of conceptual change. He later adds that child abuse "is a real evil, and it was so before the concept was constructed. It was nevertheless constructed. Neither reality nor construction should be in question" (1995, 61, 68). However, Hacking's conclusions about the reality of MPD express only a muted skepticism. Utilitarian skeptics approach this question by asking, "What is the most effective treatment?" Hacking writes that "in their hearts [such skeptics] suspect that the outcome of multiple therapy [therapy that condones or even encourages the development of multiples in the patient] is a type of false consciousness" (1995, 267). The skeptics about MPD are making a moral judgment, he believes; but on whether that moral judgment is correct, he is silent, although the suspicion must be that he agrees with it.How do these considerations apply to the case of MCI? Graham and Ritchie rightly note that classifying a subject as suffering from MCI, and informing them of the diagnosis, will affect them adversely—it will cause suffering not least because there is at present no cure. However, it must surely be conceded that any disease at all—physical or mental—is a social entity insofar as it involves behavioral ascriptions to the subject concerning their incapacity. The authors also note the vested interests—especially from the pharmaceutical industry—in developing a diagnostic category of MCI. These interests are present in the development of any significant diagnostic... (shrink)

Hume's Reception in Early America: Expanded Edition brings together the original American responses to one of Britain's greatest men of letters, David Hume. Now available as a single volume paperback, this new edition includes updated further readings suggestions and dozens of additional primary sources gathered together in a completely new concluding section. From complete pamphlets and booklets, to poems, reviews, and letters, to extracts from newspapers, religious magazines and literary and political journals, this book's contents come from a wide variety (...) of sources published in colonial America and the early United States between 1758 and 1850. As well as classics by Thomas Jefferson, John Adams and Alexander Hamilton, it contains scores of unknown and hard-to-locate items, many of which have not been reprinted since their original publication. These responses are divided into four parts covering Hume's Essays; his Philosophical Writings; his History of England; and his Character and Death. Each of those parts has a separate introductory essay, and every selection is introduced by a short headnote that sets the piece in its historical context and provides bibliographical references. Packed with new insights into Hume and American thought and culture, Hume's Reception in Early America reveals the relevance and impact of Hume on American political, philosophical, historical, religious, and aesthetic debates. (shrink)

Ludwig Wittgenstein is arguably the most important philosopher of the twentieth century. In On Certainty he discusses central issues in epistemology, including the nature of knowledge and scepticism. The Routledge Philosophy Guidebook to Wittgenstein and On Certainty introduces and assesses: Wittgenstein's career and the background to his later philosophy the central ideas and text of On Certainty , including its responses to G.E. Moore and discussion of fundamental issues in the theory of knowledge Wittgenstein's continuing importance in contemporary philosophy. This (...) GuideBook is essential reading for all students of Wittgenstein, and for those studying epistemology and philosophy of language. On Certainty, Wittgenstein's final work, addresses a category of "world-picture" propositions discovered by G.E. Moore. These challenge Wittgenstein's enduring commitment to a well-defined category of empirical propositions, and help to generate a critique of scepticism. Developing Wittgenstein's view that scepticism is self-undermining, the Guidebook offers a combative yet therapeutic interpretation that locates On Certainty between the standpoints of Kant and Hume. (shrink)

Ludwig Wittgenstein is arguably the most important philosopher of the twentieth century. In _On Certainty_ he discusses central issues in epistemology, including the nature of knowledge and scepticism. _The Routledge Philosophy Guidebook to Wittgenstein and On Certainty_ introduces and assesses: Wittgenstein's career and the background to his later philosophy the central ideas and text of _On Certainty_, including its responses to G.E. Moore and discussion of fundamental issues in the theory of knowledge Wittgenstein's continuing importance in contemporary philosophy. This _GuideBook_ (...) is essential reading for all students of Wittgenstein, and for those studying epistemology and philosophy of language. _On Certainty, _Wittgenstein's final work, addresses a category of "world-picture" propositions discovered by G.E. Moore. These challenge Wittgenstein's enduring commitment to a well-defined category of empirical propositions, and help to generate a critique of scepticism. Developing Wittgenstein's view that scepticism is self-undermining, the _Guidebook _offers a combative yet therapeutic interpretation that locates _On Certainty_ between the standpoints of Kant and Hume. (shrink)

There is a common assumption that intention is a complex behavioural disposition, or a motivational state underlying such a disposition. Associated with this position is the apparently commonsense view that an avowal of intention is a direct report of an inner motivational state, and indirectly an expression of a belief that it is likely that one will A. A central claim of this article is that the dispositional or motivational model is mistaken since it cannot acknowledge either the future-direction of (...) intention or the authority of avowals of intention. I argue that avowals of intention - first-person, present-tense ascriptions - express direct knowledge of a future action, knowledge that is not based on examination of one's present introspectible states or dispositions. Such avowals concern a future action, not a present state or disposition; just as self-ascriptions of belief concern the outer not the inner, so self-ascriptions of intention concern the future outer, not the present inner. One way of capturing this future-direction is to say that avowals of intention - and perhaps sense intentions themselves - are a kind of prediction, and not a description of one's present state of mind. This position is suggested by Anscombe in her monograph Intention (1963), and treats avowals of intention as judgements about the future, which unlike ordinary predictions are not based on evidence. However, since talk of prediction everywhere suggests an evidence-based stance - that meaningful hypothesis about the likely occurrence of events is being proposed, an hypothesis that can be falsified by evidence - the description future-outer thesis is preferred. I defend this thesis against various objections, arguing that it complements Anscombe's characterisation of intentions as based on reasons. (shrink)

This book seeks to explore relations starting from Stanley Cavell’s claim that philosophy and autobiography are dimensions of each other, first by seeking to develop a philosophy of autobiography, and then by exploring the issue from the side of six autobiographical works. This volume argues that there are good reasons for thinking that philosophical texts can be considered autobiographical, and then turns to discuss the autobiographies of Walter Benjamin, Peter Weiss, Jean-Paul Sartre, George Orwell, Edmund Gosse and Albert Camus. In (...) exploring these works, this volume explores how they put into question certain received understandings of what philosophical texts suppose themselves to be doing, and also how they themselves constitute philosophical explorations of certain key issues, e.g. the self, death, religious and ethical consciousness, sensuality, the body. Throughout, there is an exploration of the ways in which autobiographies help us in thinking about self-knowledge and knowledge of others. A final chapter raises some issues concerning the fact that the six autobiographies discussed here are all texts dealing with childhood. (shrink)

A deterministic equation of the Hamilton-Jacobi type is proposed for a single particle:S t+(1/2m)(∇S)2+U{S}=0, whereU{S} is a certain operator onS, which has the sense of the potential of the self-generated field of a free particle. Examples are given of potentials that imply instability of uniform rectilinear motion of a free particle and yieldrandom fluctuations of its trajectory. Galilei-invariant turbulence-producing potentials can be constructed using a single universal parameter—Planck's constant. Despite the fact that the classical trajectory concept is retained, the (...) mechanics of the particle then admits quantum-type effects: an uncertainty relation, de Broglie-type waves and their interference, discrete energy levels, and zero-point fluctuations. (shrink)

We are currently witnessing a renewal of broad public interest in the life and career of Alexander Hamilton – justly famed as an American founder. This volume examines the possible present-day significance of the man, noting that this is not the first revival of interest in the statesman. Hamilton was a major background figure in the GOP politics of the Gilded Age, with the powerful US Senator Henry Cabot Lodge, Sr. drawing on Hamilton to inspire a new, (...) assertive American role in the world. Hamilton was first prominent as a soldier and aide to General Washington, and believed in centralization of power in the federal government and an energetic presidency. He founded the American financial system as the first Secretary of the Treasury, and was a great moving force of America’s first nationalist-conservative party – the Federalists. As shown here, close scholarly attention to Lodge’s biography brings out the darker sides of the celebrated hero. Hamilton’s deeper conviction was the need of an elitist “aristocratic republic,” and he was an advocate of military-commercial empire. The Gilded Age Hamilton revival helped inspire the Spanish-American war of 1898 and an American overseas empire. This book will be of interest for students and professionals in political philosophy, political science, American history and American studies. (shrink)

We are currently witnessing a renewal of broad public interest in the life and career of Alexander Hamilton – justly famed as an American founder. This volume examines the possible present-day significance of the man, noting that this is not the first revival of interest in the statesman. Hamilton was a major background figure in the GOP politics of the Gilded Age, with the powerful US Senator Henry Cabot Lodge, Sr. drawing on Hamilton to inspire a new, (...) assertive American role in the world. Hamilton was first prominent as a soldier and aide to General Washington, and believed in centralization of power in the federal government and an energetic presidency. He founded the American financial system as the first Secretary of the Treasury, and was a great moving force of America’s first nationalist-conservative party – the Federalists. As shown here, close scholarly attention to Lodge’s biography brings out the darker sides of the celebrated hero. Hamilton’s deeper conviction was the need of an elitist “aristocratic republic,” and he was an advocate of military-commercial empire. The Gilded Age Hamilton revival helped inspire the Spanish-American war of 1898 and an American overseas empire. This book will be of interest for students and professionals in political philosophy, political science, American history and American studies. (shrink)

Pietraszewski asserts that social psychological research on groups is too vague, tautological, and dependent on intuitions to be theoretically useful. We disagree. Pietraszewski's contribution is thought-provoking but also incomplete and guilty of many of the faults he attributes to others. Instead of rototilling the existing knowledge landscape, we urge for more integration of new and old ideas.

Ch. 1. Filosofskoe osmyslenie kulʹtury -- ch. 2. Problemy mnozhestvennosti kulʹtur.

This book constitutes the refereed proceedings of the International Conference on Spatial Information Theory, COSIT 2005, held in Elliottville, NY, USA in September 2005. The 30 revised full papers presented were carefully reviewed and selected from 82 submissions. The papers are organized in topical sections on vagueness, uncertainty, and gradation; paths and routes; ontologies and semantics; ontologies and spatial relations; spatial reasoning: cognitive maps and spatial reasoning; time, change, and dynamics; landmarks and navigation; geographic information, and spatial behaviour.

This paper describes the topological aspect of a logic-based, artificial intelligence approach to formalising the qualitative description of spatial properties and relations, and reasoning about those properties and relations. This approach, known as RCC theory, has been under development for several years at the University of Leeds. The main rationale for this project is that qualitative descriptions of spatial properties and relationships, and qualitative spatial reasoning, are of fundamental importance in human thinking about the world: even where quantitative spatial data (...) are most important, they must be attached to the components of a perceived spatial structure if we are to make use of them. RCC theory covers other qualitative aspects of spatial description and reasoning, but the topological properties and relations of spatially extended entities are fundamental to our work. The topological formalisms used by mathematicians are, in general, not well suited to the task of formalising the kinds of ‘common-sense’ or ‘everyday’ qualitative spatial description and reasoning which are our primary interest. Nevertheless, we must come to grips with the concepts of topology as practised by mathematicians if we are not to risk constantly ‘reinventing wheels’. (shrink)

This book focuses on the political thought of American statesmen. These statesmen have had consistent and comprehensive views of the good of the country and their actions have been informed by those views. The editors argue that political life in America has been punctuated by three great crises in its history-the crisis of the Founding, the crisis of the House Divided, and the crisis of the Great Depression. The Second World War was a crisis not just for America but for (...) the whole of Western Civiliation and, in the wake of that war, a new crisis arose which came to be called the "Cold War." Just when that gave the appearance of being resolved, the world reached a new juncture, a new crisis, which Samuel P. Huntington dubbed the "clash of civiliations." The statesmen having political responsibility in confronting the first three crises in America's history came as close to philosophic grasp of the problems of liberal democracy as one could demand from those embroiled in the active resolution of events. Their reflection of political philosophy in the full sense informed their actions. Since we cannot confidently explain the future, Aristotle warned us to call no man happy while he still lives. Thus the book, in its third edition, keeps to its settled pattern of dealing with settled matters. The preface to the third edition confronts the three later crises and, to the extent consistent with truth, attempts to relate them to the first three. Morton J. Frisch was professor emeritus of political science at Northern Illinois University. He was the author or editor of several books, including Selected Writings and Speeches of Alexander Hamilton; Alexander Hamilton and the Political Order; and Franklin D. Roosevelt: The Contribution of the New Deal to American Political Thought and Practice. Richard G. Stevens retired from National Defense University as professor of political science in 1994. Since then he has taught as an adjunct professor of government at American University. He is co-editor with Matthew J. Franck of Sober as a Judge: The Supreme Court and Republican Liberty, and the author of The American Constitution and Its Provenance; Reason and History in Judicial Judgment: Felix Frankfurter and Due Process; and Political Philosophy: An Introduction. (shrink)

It was with this bitter intervention from Thrasymachus, occurring halfway through the first of its ten Books, that that work begins to come urgently alive. For the remainder of Book I the Socrates of the Dialogue asks questions and raises objections, while Thrasymachus keeps urging that in fact the just become through their very justice the victims of exploitation–the suckers!