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J. Dubcovsky [7]Jorge Dubcovsky [1]
  1. A 2600-locus chromosome bin map of wheat homoeologous group 2 reveals interstitial gene-rich islands and colinearity with rice. [REVIEW]E. J. Conley, V. Nduati, J. L. Gonzalez-Hernandez, A. Mesfin, M. Trudeau-Spanjers, S. Chao, G. R. Lazo, D. D. Hummel, O. D. Anderson, L. L. Qi, B. S. Gill, B. Echalier, A. M. Linkiewicz, J. Dubcovsky, E. D. Akhunov, J. Dvořák, J. H. Peng, N. L. V. Lapitan, M. S. Pathan, H. T. Nguyen, X. -F. Ma, Miftahudin, J. P. Gustafson, R. A. Greene, M. E. Sorrells, K. G. Hossain, V. Kalavacharla, S. F. Kianian, D. Sidhu, M. Dilbirligi, K. S. Gill, D. W. Choi, R. D. Fenton, T. J. Close, P. E. McGuire, C. O. Qualset & J. A. Anderson - unknown
    The complex hexaploid wheat genome offers many challenges for genomics research. Expressed sequence tags facilitate the analysis of gene-coding regions and provide a rich source of molecular markers for mapping and comparison with model organisms. The objectives of this study were to construct a high-density EST chromosome bin map of wheat homoeologous group 2 chromosomes to determine the distribution of ESTs, construct a consensus map of group 2 ESTs, investigate synteny, examine patterns of duplication, and assess the colinearity with rice (...)
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  2. Group 3 chromosome bin maps of wheat and their relationship to rice chromosome 1.J. D. Munkvold, R. A. Greene, C. E. Bermudez-Kandianis, C. M. La Rota, H. Edwards, S. F. Sorrells, T. Dake, D. Benscher, R. Kantety, A. M. Linkiewicz, J. Dubcovsky, E. D. Akhunov, J. Dvořák, Miftahudin, J. P. Gustafson, M. S. Pathan, H. T. Nguyen, D. E. Matthews, S. Chao, G. R. Lazo, D. D. Hummel, O. D. Anderson, J. A. Anderson, J. L. Gonzalez-Hernandez, J. H. Peng, N. Lapitan, L. L. Qi, B. Echalier, B. S. Gill, K. G. Hossain, V. Kalavacharla, S. F. Kianian, D. Sandhu, M. Erayman, K. S. Gill, P. E. McGuire, C. O. Qualset & M. E. Sorrells - unknown
    The focus of this study was to analyze the content, distribution, and comparative genome relationships of 996 chromosome bin-mapped expressed sequence tags accounting for 2266 restriction fragments on the homoeologous group 3 chromosomes of hexaploid wheat. Of these loci, 634, 884, and 748 were mapped on chromosomes 3A, 3B, and 3D, respectively. The individual chromosome bin maps revealed bins with a high density of mapped ESTs in the distal region and bins of low density in the proximal region of the (...)
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  3. Chromosome bin map of expressed sequence tags in homoeologous group 1 of hexaploid wheat and homoeology with rice and arabidopsis.J. H. Peng, H. Zadeh, G. R. Lazo, J. P. Gustafson, S. Chao, O. D. Anderson, L. L. Qi, B. Echalier, B. S. Gill, M. Dilbirligi, D. Sandhu, K. S. Gill, R. A. Greene, M. E. Sorrells, E. D. Akhunov, J. Dvořák, A. M. Linkiewicz, J. Dubcovsky, K. G. Hossain, V. Kalavacharla, S. F. Kianian, A. A. Mahmoud, Miftahudin, E. J. Conley, J. A. Anderson, M. S. Pathan, H. T. Nguyen, P. E. McGuire, C. O. Qualset & N. L. V. Lapitan - unknown
    A total of 944 expressed sequence tags generated 2212 EST loci mapped to homoeologous group 1 chromosomes in hexaploid wheat. EST deletion maps and the consensus map of group 1 chromosomes were constructed to show EST distribution. EST loci were unevenly distributed among chromosomes 1A, 1B, and ID with 660, 826, and 726, respectively. The number of EST loci was greater on the long arms than on the short arms for all three chromosomes. The distribution of ESTs along chromosome arms (...)
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  4. Deletion mapping of homoeologous group 6-specific wheat expressed sequence tags.H. S. Randhawa, M. Dilbirligi, D. Sidhu, M. Erayman, D. Sandhu, S. Bondareva, S. Chao, G. R. Lazo, O. D. Anderson, Miftahudin, J. P. Gustafson, B. Echalier, L. L. Qi, B. S. Gill, E. D. Akhunov, J. Dvořák, A. M. Linkiewicz, A. Ratnasiri, J. Dubcovsky, C. E. Bermudez-Kandianis, R. A. Greene, M. E. Sorrells, E. J. Conley, J. A. Anderson, J. H. Peng, N. L. V. Lapitan, K. G. Hossain, V. Kalavacharla, S. F. Kianian, M. S. Pathan, H. T. Nguyen, T. R. Endo, T. J. Close, P. E. McGuire, C. O. Qualset & K. S. Gill - unknown
    To localize wheat ESTs on chromosomes, 882 homoeologous group 6-specific ESTs were identified by physically mapping 7965 singletons from 37 cDNA libraries on 146 chromosome, arm, and sub-arm aneuploid and deletion stocks. The 882 ESTs were physically mapped to 25 regions flanked by 23 deletion breakpoints. Of the 5154 restriction fragments detected by 882 ESTs, 2043 were localized to group 6 chromosomes and 806 were mapped on other chromosome groups. The number of loci mapped was greatest on chromosome 6B and (...)
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  5.  28
    Analysis of expressed sequence tag loci on wheat chromosome group 4. Miftahudin, K. Ross, X. -F. Ma, A. A. Mahmoud, J. Layton, M. A. Rodriguez Milla, T. Chikmawati, J. Ramalingam, O. Feril, M. S. Pathan, G. Surlan Momirovic, S. Kim, K. Chema, P. Fang, L. Haule, H. Struxness, J. Birkes, C. Yaghoubian, R. Skinner, J. McAllister, V. Nguyen, L. L. Qi, B. Echalier, B. S. Gill, A. M. Linkiewicz, J. Dubcovsky, E. D. Akhunov, J. Dvořák, M. Dilbirligi, K. S. Gill, J. H. Peng, N. L. V. Lapitan, C. E. Bermudez-Kandianis, M. E. Sorrells, K. G. Hossain, V. Kalavacharla, S. F. Kianian, G. R. Lazo, S. Chao, O. D. Anderson, J. Gonzalez-Hernandez, E. J. Conley, J. A. Anderson, D. -W. Choi, R. D. Fenton, T. J. Close, P. E. McGuire, C. O. Qualset, H. T. Nguyen & J. P. Gustafson - unknown
    A total of 1918 loci, detected by the hybridization of 938 expressed sequence tag unigenes from 26 Triticeae cDNA libraries, were mapped to wheat homoeologous group 4 chromosomes using a set of deletion, ditelosomic, and nulli-tetrasomic lines. The 1918 EST loci were not distributed uniformly among the three group 4 chromosomes; 41, 28, and 31% mapped to chromosomes 4A, 4B, and 4D, respectively. This pattern is in contrast to the cumulative results of EST mapping in all homoeologous groups, as reported (...)
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  6.  1
    A chromosome bin map of 2148 expressed sequence tag loci of wheat homoeologous group 7.K. G. Hossain, V. Kalavacharla, G. R. Lazo, J. Hegstad, M. J. Wentz, P. M. A. Kianian, K. Simons, S. Gehlhar, J. L. Rust, R. R. Syamala, K. Obeori, S. Bhamidimarri, P. Karunadharma, S. Chao, O. D. Anderson, L. L. Qi, B. Echalier, B. S. Gill, A. M. Linkiewicz, A. Ratnasiri, J. Dubcovsky, E. D. Akhunov, J. Dvořák, Miftahudin, K. Ross, J. P. Gustafson, H. S. Radhawa, M. Dilbirligi, K. S. Gill, J. H. Peng, N. L. V. Lapitan, R. A. Greene, C. E. Bermudez-Kandianis, M. E. Sorrells, O. Feril, M. S. Pathan, H. T. Nguyen, J. L. Gonzalez-Hernandez, E. J. Conley, J. A. Anderson, D. W. Choi, D. Fenton, T. J. Close, P. E. McGuire, C. O. Qualset & S. F. Kianian - unknown
    The objectives of this study were to develop a high-density chromosome bin map of homoeologous group 7 in hexaploid wheat, to identify gene distribution in these chromosomes, and to perform comparative studies of wheat with rice and barley. We mapped 2148 loci from 919 EST clones onto group 7 chromosomes of wheat. In the majority of cases the numbers of loci were significantly lower in the centromeric regions and tended to increase in the distal regions. The level of duplicated loci (...)
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  7.  1
    Development of an expressed sequence tag resource for wheat : EST generation, unigene analysis, probe selection and bioinformatics for a 16,000-locus bin-delineated map. [REVIEW]G. R. Lazo, S. Chao, D. D. Hummel, H. Edwards, C. C. Crossman, N. Lui, D. E. Matthews, V. L. Carollo, D. L. Hane, F. M. You, G. E. Butler, R. E. Miller, T. J. Close, J. H. Peng, N. L. V. Lapitan, J. P. Gustafson, L. L. Qi, B. Echalier, B. S. Gill, M. Dilbirligi, H. S. Randhawa, K. S. Gill, R. A. Greene, M. E. Sorrells, E. D. Akhunov, J. Dvořák, A. M. Linkiewicz, J. Dubcovsky, K. G. Hossain, V. Kalavacharla, S. F. Kianian, A. A. Mahmoud, Miftahudin, X. -F. Ma, E. J. Conley, J. A. Anderson, M. S. Pathan, H. T. Nguyen, P. E. McGuire, C. O. Qualset & O. D. Anderson - unknown
    This report describes the rationale, approaches, organization, and resource development leading to a large-scale deletion bin map of the hexaploid wheat genome. Accompanying reports in this issue detail results from chromosome bin-mapping of expressed sequence tags representing genes onto the seven homoeologous chromosome groups and a global analysis of the entire mapped wheat EST data set. Among the resources developed were the first extensive public wheat EST collection. Described are protocols for sequencing, sequence processing, EST nomenclature, and the assembly of (...)
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