An autobiographical account of the liposome, from the perplexities of a blood smear to the growth of a multi‐million pound business.

A new design principle is discussed for making a sufficiently complex cell for the creation of the first wet artificial life in the laboratory. The current approach is to attempt a minimal cell, which consists of a liposome that contains a minimal metabolic cycle for self-maintenance and self-replication. Given the lack of success with the minimal cell to date, the authors suggest it is possible to take an alternative approach to building the first wet artificial life form that they have (...) called the first cell. In this article, the concept of the first cell versus the minimal cell is discussed. The new design principle is supported by the following observations that are examined and developed from an Origins of Life perspective: That human development shows a U-shaped curve where the skills and developmental patterns of human infants are proficient at the first phase then decline and subsequently recover to a qualitatively better level. A review of spatial temporal dynamics using cellular automata studies shows that complexity generated at both the initial states and the time evolution dynamics can synthesize rich evolutionary processes. A chemical experiment exhibiting self-organizing dynamics results in the emergence of a self-propelling oil droplet that demonstrates the self-sustaining properties of a non-equilibrium state. Finally, the important design question Is life a contingent or deterministic phenomenon? is examined which asks whether a life form is a dynamic product of its surroundings or whether it is the inevitable outcome of prior events, and is discussed making reference to the characteristics of an art installation The Way Things Go. This contemporary masterpiece, which exhibits a long-lasting chain of unrelated causal events, serves as a theoretical model for discussion of evolutionary processes, suggesting that when it comes to designing wet artificial life forms in the laboratory, both contingent and deterministic processes should be taken into account. (shrink)

The demonstration that dendritic cell (DC)‐derived exosomes can be exploited for targeted RNAi delivery to the brain after systemic injection provides the first proof‐of‐concept for the potential of these naturally occurring vesicles as vehicles of drug delivery. As well as being amenable to existing in vivo targeting strategies already in use for viruses and liposomes, this novel approach offers the added advantages of in vivo safety and low immunogenicity. Fulfilment of the potential of exosome delivery methods warrants a better (...) understanding of their biology, as well as the development of novel production, characterisation, targeting and cargo‐loading nanotechnologies. Ultimately, exosome‐mediated drug delivery promises to overcome important challenges in the field of therapeutics, such as delivery of drugs across otherwise impermeable biological barriers, such as the blood brain barrier, and using patient‐derived tissue as a source of individualised and biocompatible therapeutic drug delivery vehicles. (shrink)

Recent work by Fernández‐Sánchez and coworkers examining the impact of applied pressure on the malignant phenotype of murine colon tissue in vivo revealed that mechanical perturbations can drive malignant behavior in genetically normal cells. Their findings build upon an existing understanding of how the mechanical cues experienced by cells within a tissue become progressively modified as the tissue transforms. Using magnetically stimulated ultra‐magnetic liposomes to mimic tumor growth ‐induced solid stress, Fernández‐Sánchez and coworkers were able to stimulate β‐catenin to (...) promote the cancerous behavior of both a normal and genetically modified colon epithelium. In this perspective, we discuss their findings in the context of what is currently known regarding the role of the mechanical landscape in cancer progression and β‐catenin as a mechanotransducer. We review data that suggest that mechanically regulated activation of β‐catenin fosters development of a malignant phenotype in tissue and predict that mechanical cues may contribute to tumor heterogeneity. (shrink)

The recent development of RNA replicating protocells and capsules that enclose complex biosynthetic cascade reactions are encouraging signs that we are gradually getting better at mastering the complexity of biological systems. The road to truly cellular compartments is still very long, but concrete progress is being made. Compartmentalization is a crucial natural methodology to enable control over biological processes occurring within the living cell. In fact, compartmentalization has been considered by some theories to be instrumental in the creation of life. (...) With the advancement of chemical biology, artificial compartments that can mimic the cell as a whole, or that can be regarded as cell organelles, have recently received much attention. The membrane between the inner and outer environment of the compartment has to meet specific requirements, such as semi‐permeability, to allow communication and molecular transport over the border. The membrane can either be built from natural constituents or from synthetic polymers, introducing robustness to the capsule. (shrink)

Lipid vesicles are often used as compartment structures for preparing cell‐like systems and models of protocells, the hypothetical precursor structures of the first cells at the origin of life. Although the various artificially made vesicle systems are already remarkably complex, they are still very different from and much simpler than any known living cell. Nevertheless, the preparation and study of the structure and the dynamics of functionalized vesicle systems may contribute to a better understanding of biological cells, in particular of (...) the essential features of a living cell that are not found in the non‐living form of matter. The study of protocell models may possibly lead to a better understanding of the origin of the first cells. To avoid misunderstanding in this field of research, it would be useful if generally accepted definitions of terms like “artificial cells,” “synthetic cells,” “minimal cells,” “protocells,” and “primitive cells” exist. Editor's suggested further reading in BioEssaysSynthetic cells and organelles: compartmentalization strategies Abstract. (shrink)