Stereological techniques allow biologists to create quantitative, three-dimensional descriptions of biological structures from two- dimensional images of tissue viewed under the microscope. For example, they can accurately estimate the size of a particular organelle, the total length of a mass of capillaries, or the number of neurons or synapses in a particular region of the brain. This book provides a practical guide to designing and critically evaluating stereological studies of the nervous system and other tissues. It explains the basic concepts (...) behind design-based stereology and how to get started. Also included are detailed descriptions of how to prepare tissue appropriately, perform pilot studies and decide on the appropriate sampling strategy, and account for phenomena such as tissue shrinkage. Numerous examples of applications of stereological methods that are applicable to studies of the central system and a wide variety of other tissues are explained. The book is therefore essential reading for neurobiologists and cell biologists interested in generating accurate representations of cell and tissue architecture. (shrink)

In Unbiased Stereology, the authors present a practical guide to making unbiased 3D measurements using microscopy. Stereology is the statistical inference of geometric parameters from spatially sampled information; it is considered unbiased when the test probes are positioned in an appropriately random manner. Now that unbiased stereology is required for nerve-cell counts in neurotoxicology by the FDA and by many journals before the acceptance of an article or study, this book is essential reading. It includes only those (...) stereological techniques that have been tried and tested by actual application. Specific topics covered include: random sampling and random geometry; the estimation of feature volume, surface, length, number and mean particle size; as well as statistics for stereologists. (shrink)

The Advanced Methods series is intented for advanced undergraduates, postgraduates and established research scientists. Titles in the series are designed to cover current important areas of research in life sciences, and include both theoretical background and detailed protocols. The aim is to give researchers sufficient theory, supported by references, to take the given protocols and adapt them to their particular experimental systems. Unbiased Stereology , Second Edition expands the comprehensive practical first edition guide to 3-D measurements in microscopy using (...) stereological methods by adding a section on 2-D stereology, or "Petri-metrics". There is also an additional chapter on single-object stereology, which permits the measurement of total volume, surface and feature length of single arbitrary objects. The book is intended to lead the reader logically through a self-teaching course, with illustrative exercises at each stage. The techniques described have become benchmark methods in toxicology and neuroscience; the use of inferior methods often leading to missed or spurious results. (shrink)

Stereology is a valuable tool for neuroscientists, allowing them to obtain 3-Dimensional information from 2-Dimensional measurements made on appropriately sampled sections. This 3-D information is invaluable in correlating structural/functional relationships in the pursuit of far greater understanding of the function of the central nervous system. However, in carrying out such measurements, often based on limited data sets, there is a risk of experimenter bias. An important feature of modern design based stereology is to be aware of potential sources (...) of bias and eliminate them during the data collection. With many of the major neuroscience journals now insisting that quantitative data be presented, there is a greater need than ever for neuroscientists to understand the theory and practice behind quantitative methods, such as those offered by stereology. Quantitative Methods in Neuroscience is a cookbook of stereological methods written especially for neuroscientists. It provides clear and accessible advice about when and when not to use stereology. Throughout the book, the emphasis is on practical guidance, rather than discussions and formulae. Written by leading scientists in the field of stereology, with a Foreword by D.C. Sterio, the book will be a valuable introduction to these methods for neuroscientists, and all those involved in development of new drug programmes.Readership: Neuroscientists doing quantitative studies; Scientists involved in drug development; Pathologists. (shrink)

A timely collection of advanced, original material in the area of statistical methodology motivated by geometric problems, dedicated to the influential work of Kanti V. Mardia This volume celebrates Kanti V. Mardia's long and influential career in statistics. A common theme unifying much of Mardia’s work is the importance of geometry in statistics, and to highlight the areas emphasized in his research this book brings together 16 contributions from high-profile researchers in the field. Geometry Driven Statistics covers a wide range (...) of application areas including directional data, shape analysis, spatial data, climate science, fingerprints, image analysis, computer vision and bioinformatics. The book will appeal to statisticians and others with an interest in data motivated by geometric considerations. Summarizing the state of the art, examining some new developments and presenting a vision for the future, Geometry Driven Statistics will enable the reader to broaden knowledge of important research areas in statistics and gain a new appreciation of the work and influence of Kanti V. Mardia. (shrink)

Increasingly, functional and evolutionary research has highlighted the important contribution emotion processing makes to complex human social cognition. As such, it may be asked whether neural structures involved in emotion processing, commonly referred to as limbic structures, have been impacted in human brain evolution. To address this question, we performed an extensive evolutionary analysis of multiple limbic structures using modern phylogenetic tools. For this analysis, we combined new volumetric data for the hominoid (human and ape) amygdala and 4 amygdaloid nuclei, (...) hippocampus, and striatum, collected using stereological methods in complete histological series, with previously published datasets on the amygdala, orbital and medial frontal cortex, and insula, as well as a non-limbic structure, the dorsal frontal cortex, for contrast. We performed a parallel analysis using large published datasets including many anthropoid species (human, ape, and monkey), but fewer hominoids, for the amygdala and 2 amygdaloid subdivisions, hippocampus, schizocortex, striatum, and septal nuclei. To address evolutionary change, we compared observed human values to values predicted from regressions run through a) nonhuman hominoids and b) nonhuman anthropoids, assessing phylogenetic influence using phylogenetic generalized least squares regression.Compared with other hominoids, the volumes of the hippocampus, the lateral nucleus of the amygdala, and the orbital frontal cortex were, respectively, 50%, 37%, and 11% greater in humans than predicted for an ape of human hemisphere volume, while the medial and dorsal frontal cortex were, respectively, 26% and 29% significantly smaller. Compared with other anthropoids, only human values for the striatum fell significantly below predicted values. Overall, the data present support for the idea that regions involved in emotion processing are not necessarily conserved or regressive, but may even be enhanced in recent human evolution. (shrink)

According to the author, no body of theories deserves to be called philosophy unless some of these theories and problems fall within the province of metphysics. No problems deserves the name of metaphysics unless some of them add up to constitute ontology. The author presents the reistic version of the science of being as the union of Protothetic, Ontology, Mereology and Chronology. The next possible step in the construction of the reistic ontology will be Stereology (a kind of reistic (...) geometry). (shrink)

In the world of globalization religious-artistic works remain a phenomenon study of which allows to observe the main tendencies of socio-cultural dynamics taking into account complicated and multi-plan contexts of its realization. Methodological peculiarities of the suggested approach base on philosophic comparative study and interdisciplinary method, which allow neutralizing negative consequences of scientist approach based on physiological–ideological projectivity. In this case correlation of sacral and aesthetic works as crossing of “vertical” and “horizontal” dimensions which opens “the second derivative” of thecreative (...) process – its Stereological orientation. At the threshold of the second decade of the 21 century it is expedient to activate comparative analysis of the problem of creation through the prism of cultures of various types at the specific material. This will allow us to understand more fully opportunities of global strategy of unity of the humanity on the basis of great achievements of culture having determined its boundaries and perspectives. (shrink)

In the world of globalization religious-artistic works remain a phenomenon study of which allows to observe the main tendencies of socio-cultural dynamics taking into account complicated and multi-plan contexts of its realization. Methodological peculiarities of the suggested approach base on philosophic comparative study and interdisciplinary method, which allow neutralizing negative consequences of scientist approach based on physiological–ideological projectivity. In this case correlation of sacral and aesthetic works as crossing of “vertical” and “horizontal” dimensions which opens “the second derivative” of thecreative (...) process – its Stereological orientation. At the threshold of the second decade of the 21 century it is expedient to activate comparative analysis of the problem of creation through the prism of cultures of various types at the specific material. This will allow us to understand more fully opportunities of global strategy of unity of the humanity on the basis of great achievements of culture having determined its boundaries and perspectives. (shrink)