Aim of this study was to investigate whether parental mental representations during pregnancy and after delivery differed between parents who conceived after Assisted Reproductive Treatments and spontaneous conceiving parents. Effects of specific ART variables were also taken into account. Seventeen ART couples and 25 SC couples were recruited at Santa Maria Nuova Hospital. At both 32 weeks of gestation and 3 months postpartum participants completed the Semantic Differential of the IRMAG, a self-report tool which measures specific domains of mental representations (...) pertaining either individual or parental characteristics. Results showed that ART parents had significantly more positive representations of the child compared to SC parents, while the scores at Partner dimension improved from T1 to T2 for SC parents only. With regards to ART history, scores at the Self-as-woman/man dimension were significantly less positive for ICSI than IVF parents and improved substantially from T1 to T2 only in case of mothers with previous ART attempts and of fathers at the first ART cycle. The representation of own parents increased from T1 to T2 in case of infertility diagnosis due to male factors, while a decrease emerged when infertility was due to female factors. Findings suggest the need to investigate parental mental representations after ART, in order to improve the understanding on the transition to parenthood of infertile couples and to target more specific intervention for parenting support. (shrink)