Abstract

Estimation of the repartition of asynchronous cells in the cell cycle can be explained by two hypotheses:– - the cells are supposed to be distributed into three groups: cells with a 2c DNA content (G0/1 phase), cells with a 4c DNA content (G2+M phase) and cells with a DNA content ranging from 2c to 4c (S phase); – - there is a linear relationship between the amount of fluorescence emitted by the fluorescent probe which reveals the DNA and the DNA content. According to these hypotheses, the cell cycle can be represented by the following equation.