Tissues contain complex populations of cells. Like countries, which are comprised of mixed populations of people, tissues are not homogeneous. Gene expression studies that analyze entire populations of cells from tissues as a mixture are blind to this diversity. Thus, critical information is lost when studying samples rich in specialized but diverse cells such as tumors, iPS colonies, or brain tissue. High throughput methods are needed to address, model and understand the constitutive and stochastic differences between individual cells. Here, we (...) describe microfluidics technologies that utilize a combination of molecular biology and miniaturized labs on chips to study gene expression at the single cell level. We discuss how the characterization of the transcriptome of each cell in a sample will open a new field in gene expression analysis, population transcriptomics, that will change the academic and biomedical analysis of complex samples by defining them as quantified populations of single cells. (shrink)

The apologetic method of the Nyāya is inductive. The subject is distilled from the scripture and the Nyāya investigates it through logical argument. Through this procedure of partial verification, the reliability of the composer is established, and consequently the authority of the entire scripture is justified. The domains of thescripture and rational investigation overlap in significant issues such as the reality of the Self, and therefore, they can cooperate to establish the common truth. In Mīmāṃsā apologetics, the domain of the (...) scripture and that of rational argument are mutually exclusive. Their apologetic program is basically accomplished as a process of purely rational investigation without referring to the content of the scripture. But at the same time, the authority of the Veda is regarded as innate and has no external basis. Working autonomously, the rational investigation imposes its own limitation from within and thereby secures the scriptural domain that is free from the intervention of any other kind of knowledge. The overlap between the domains of the scripture and the ordinary experience including rational investigation constitutes a necessary condition for the Nyāya apologetics, whereas in Mīmāṃsā apologetics, it is the absence of the overlapping domain that assures the unique authority of the Veda. This difference is supposed to be rooted in their disagreement on whether a man is able, or qualified, to examine the content of the scripture or not. (shrink)

江戸時代中期に開学した漢学塾懐徳堂。中井竹山・履軒兄弟が展開した懐徳堂儒学と朱子学との関係、思想史的意義を明らかにする。.

Own-age bias is a well-known bias reflecting the effects of age, and its role has been demonstrated, particularly, in face recognition. However, it remains unclear whether an own-age bias exists in facial impression formation. In the present study, we used three datasets from two published and one unpublished functional magnetic resonance imaging study that employed the same pleasantness rating task with fMRI scanning and preferential choice task after the fMRI to investigate whether healthy young and older participants showed own-age effects (...) in face preference. Specifically, we employed a drift-diffusion model to elaborate the existence of own-age bias in the processes of preferential choice. The behavioral results showed higher rating scores and higher drift rate for young faces than for older faces, regardless of the ages of participants. We identified a young-age effect, but not an own-age effect. Neuroimaging results from aggregation analysis of the three datasets suggest a possibility that the ventromedial prefrontal cortex was associated with evidence accumulation of own-age faces; however, no clear evidence was provided. Importantly, we found no age-related decline in the responsiveness of the vmPFC to subjective pleasantness of faces, and both young and older participants showed a contribution of the vmPFC to the parametric representation of the subjective value of face and functional coupling between the vmPFC and ventral visual area, which reflects face preference. These results suggest that the preferential choice of face is less susceptible to the own-age bias across the lifespan of individuals. (shrink)

Stuttering is a neurodevelopmental speech disorder characterized by the symptoms of speech repetition, prolongation, and blocking. Stuttering-related dysfluency can be transiently alleviated by providing an external timing signal such as a metronome or the voice of another person. Therefore, the existence of a core motor timing deficit in stuttering has been speculated. If this is the case, then motoric behaviors other than speech should be disrupted in stuttering. This study examined motoric performance on four complex bimanual tasks in 37 adults (...) who stutter and 31 fluent controls. Two tasks utilized bimanual rotation to examine motor dexterity, and two tasks used the bimanual mirror and parallel tapping movements to examine timing control ability. Video-based analyses were conducted to determine performance accuracy and speed. The results showed that individuals who stutter performed worse than fluent speakers on tapping tasks but not on bimanual rotation tasks. These results suggest stuttering is associated with timing control for general motor behavior. (shrink)