Macromolecular interactions play a central role in many biological processes. Protein‐protein interactions have mostly been studied by co‐immunoprecipitation, which cannot provide quantitative information on all possible molecular connections present in the complex. We will review a new approach that allows cellular proteins and biomolecular complexes to be studied in real‐time at the single‐molecule level. This technique is called single‐molecule pull‐down (SiMPull), because it integrates principles of conventional immunoprecipitation with the powerful single‐molecule fluorescence microscopy. SiMPull is used to count how many (...) of each protein is present in the physiological complexes found in cytosol and membranes. Concurrently, it serves as a single‐molecule biochemical tool to perform functional studies on the pulled‐down proteins. In this review, we will focus on the detailed methodology of SiMPull, its salient features and a wide range of biological applications in comparison with other biosensing tools. (shrink)

The twenty-one chapters in this volume strive, through the use of high quality argument and analysis, to get a good deal clearer concerning a range of issues ...

Defense of rationality ; precedence of reason over religion.

Religion and philosophy in 'Akāla usatati', portion of Daswen̲ Pādshāh kā Granth, attributed to Gobind Singh, Guru, 1666-1708.

Conversation with Navjyoti Singh, philosopher, chiefly about his views of modern Indic philosophy.

Pascal’s Wager is simply too good to be true—or better, too good to be sound. There must be something wrong with Pascal’s argument that decision-theoretic reasoning shows that one must (resolve to) believe in God, if one is rational. No surprise, then, that critics of the argument are easily found, or that they have attacked it on many fronts. For Pascal has given them no dearth of targets.

Pascal’s Wager is simply too good to be true—or better, too good to be sound. There must be something wrong with Pascal’s argument that decision-theoretic reasoning shows that one must believe in God, if one is rational. No surprise, then, that critics of the argument are easily found, or that they have attacked it on many fronts. For Pascal has given them no dearth of targets.

Commentary on Śārīrakamīmāṃsābhāṣya of Śaṅkarācārya, work on Vedanta philosophy.

This book is a festschrift for Torsten Hagerstrand. "Through your work on migration, innovation diffusion, and time-geography you have helped demonstrate that geography's most profound insights are to be gained from the study of process rather than form.".

In the first part of this paper we investigate the intuitionistic version $iI\!\Sigma_1$ of $I\!\Sigma_1$ (in the language of $PRA$ ), using Kleene's recursive realizability techniques. Our treatment closely parallels the usual one for $HA$ and establishes a number of nice properties for $iI\!\Sigma_1$ , e.g. existence of primitive recursive choice functions (this is established by different means also in [D94]). We then sharpen an unpublished theorem of Visser's to the effect that quantifier alternation alone is much less powerful intuitionistically (...) than classically: $iI\!\Sigma_1$ together with induction over arbitrary prenex formulas is $\Pi_2$ -conservative over $iI\!\Pi_2$ . In the second part of the article we study the relation of $iI\!\Sigma_1$ to $iI\!\Pi_1$ (in the usual arithmetical language). The situation here is markedly different from the classical case in that $iI\!\Pi_1$ and $iI\!\Sigma_1$ are mutually incomparable, while $iI\!\Sigma_1$ is significantly stronger than $iI\!\Pi_1$ as far as provably recursive functions are concerned: All primitive recursive functions can be proved total in $iI\!\Sigma_1$ whereas the provably recursive functions of $iI\!\Pi_1$ are all majorized by polynomials over ${\Bbb N}$ . 0 $iI\!\Pi_1$ is unusual also in that it lacks closure under Markov's Rule $\mbox{MR}_{PR}$. (shrink)

Should we make people healthier, smarter, and longer-lived if genetic and medical advances enable us to do so? Matti Häyry asks this question in the context of genetic testing and selection, cloning and stem cell research, gene therapies and enhancements. The ethical questions explored include parental responsibility, the use of people as means, the role of hope and fear in risk assessment, and the dignity and meaning of life. Taking as a starting point the arguments presented by Jonathan Glover, John (...) Harris, Ronald M. Green, Jürgen Habermas, Michael J. Sandel, and Leon R. Kass, who defend a particular normative view as the only rational or moral answer, Matti Häyry argues that many coherent rationalities and moralities exist in the field, and that to claim otherwise is mistaken"--Provided by publisher. (shrink)

A profound, original, and accessible book that argues that a faith not in God or eternal life, but in the finite, temporal life we lead here on earth is one that gives that life far greater depth of meaning. A manifesto for a truly secular faith that speaks eloquently to both believers and agnostics alike. The philosopher and critic Martin Hägglund believes that we need a new way of thinking about faith. In contrast to the traditional religious faith in eternity, (...) he proposes a secular faith in the value of living in time. He argues that the concept of an eternal heaven actually renders our mortal life meaningless since it assumes that our ultimate aims should be to escape it. Engaging writers and thinkers as diverse as C. S. Lewis, Kierkegaard, St. Augustine, Nietzsche, Martin Luther, and even Karl Ove Knausgaard, Hägglund provides not only a critique of religious ideals, but also a positive, alternative understanding of the beliefs and values that can motivate us to live lives of meaning in the here and now. (shrink)

This book is an investigation into the descriptive task of moral philosophy. Nora Hämäläinen explores the challenge of providing rich and accurate pictures of the moral conditions, values, virtues, and norms under which people live and have lived, along with relevant knowledge about the human animal and human nature. While modern moral philosophy has focused its energies on normative and metaethical theory, the task of describing, uncovering, and inquiring into moral frameworks and moral practices has mainly been left to social (...) scientists and historians. Nora Hämäläinen argues that this division of labour has detrimental consequences for moral philosophy and that a reorientation toward descriptive work is needed in moral philosophy. She traces resources for a descriptive philosophical ethics in the work of four prominent philosophers of the twentieth century: John Dewey, Ludwig Wittgenstein, Michel Foucault, and Charles Taylor, while also calling on thinkers inspired by them. (shrink)

On the concept of sense in the philosophy of Carvaka, exponent of the materialistic school in Indic philosophy.

_Radical Atheism_ presents a profound new reading of the influential French philosopher Jacques Derrida. Against the prevalent notion that there was an ethical or religious "turn" in Derrida's thinking, Hägglund argues that a radical atheism informs Derrida's work from beginning to end. Proceeding from Derrida's insight into the constitution of time, Hägglund demonstrates how Derrida rethinks the condition of identity, ethics, religion, and political emancipation in accordance with the logic of radical atheism. Hägglund challenges other major interpreters of Derrida's work (...) and offers a compelling account of Derrida's thinking on life and death, good and evil, self and other. Furthermore, Hägglund does not only explicate Derrida's position but also develops his arguments, fortifies his logic, and pursues its implications. The result is a groundbreaking deconstruction of the perennial philosophical themes of time and desire as well as pressing contemporary issues of sovereignty and democracy. (shrink)

First published in 2002. Routledge is an imprint of Taylor & Francis, an informa company.

This book explores the place of the sophists within the Greek wisdom tradition, and argues against their almost universal exclusion from serious intellectual ...