The gene regulation function (GRF) provides an operational description of a promoter behavior as a function of the concentration of one of its transcriptional regulators. Behind this apparently trivial definition lies a central concept in biological control: the GRF provides the input/output relationship of each edge in a transcriptional network, independently from the molecular interactions involved. Here we discuss how existing methods allow direct measurement of the GRF, and how several trade‐offs between scalability and accuracy have hindered its application to (...) relatively large networks. We discuss the theoretical and technical requirements for obtaining the GRF. Based on these requirements, we introduce a simplified and easily scalable method that is able to capture the significant parameters of the GRF. The GRF is able to predict the behavior of a simple genetic circuit, illustrating how addressing the quantitative nature of gene regulation substantially increases our comprehension on the mechanisms of gene control. (shrink)

Today the definition of Spain as a national, historical and sovereign entity is in crisis. This situation has been reached because the Spanish left has assumed as its own many of the ideas about the concept of the Spanish nation of peripheral nationalisms. In this article we try to discover the causes of this identification through the study of the propaganda texts that the opposition to the Franco regime had on the national concept, rescued in the rulings of the Public (...) Order Court. In them we will observe how the opposition to Francoism in the Basque Country, Catalonia, Galicia and the Canary Islands mixed Marxist and nationalist ideologies in their messages, but with great fundamental differences in each of these regions. Furthermore, we certify that the "Burgos Process" was the turning point that cemented the identification of the Spanish left throughout the country with the nationalist movements. (shrink)