This year marks the 60th year anniversary of the publication of Niko Tinbergen’s “On aims and methods of ethology” which remains influential among today’s biologists and social scientists for its introduction of four questions for a complete explanation for animal behaviors. In this paper we argue that a large part of the lasting appeal to Tinbergen’s four questions was (and still is) the methodological commitment to treating organisms as objects as opposed to purposive agents. Tinbergen’s approach reinvigorated the discipline of (...) ethology, allowing it to shed its teleological and anthropomorphic associations and to better cohere with a philosophy of science that favors inductive procedures, causal and mechanistic analytic techniques, and an emphasis on Darwinian explanations. While Tinbergen’s approach is still prized among today’s biological social scientists, it ignores an important feature of many social organisms, that they are not merely objects, they are also purposive agents. We explore the implications that a shift from treating organisms as objects to treating them as agents has on both how we should interpret and answer Tinbergen’s four questions. Updating Tinbergen’s four questions with agency in mind not only makes them more applicable to the biological investigation of animal behavior, but also strengthens the value and applicability of biology-oriented research programs in the social sciences. (shrink)