Based on the life history theory and broadening construction theory, the study aimed to investigate the influence of Machiavellianism on the learning-related subjective well-being and the underlying mechanism, 582 Chinese senior high school students including 289 girls and 310 boys were recruited to participate in this study, and they anonymously filled out questionnaires regarding Machiavellianism, learning-related subjective well-being, gratitude, and subjective family economic level. The results showed that: a higher level of Machiavellianism was associated with a lower level of learning-related (...) subjective well-being; gratitude partially mediated the relationship between Machiavellianism and learning-related subjective well-being; subjective family economic level moderated the links between Machiavellianism and learning-related subjective well-being, and between gratitude and learning-related subjective well-being. This study explained how and when Machiavellianism affected Chinese senior high school students’ learning-related subjective well-being and provided a deeper understanding of the relationship between Machiavellianism and learning-related subjective well-being. (shrink)