Innovative loss‐of‐function techniques developed in recent years have made it much easier to target specific genomic loci at transcriptional levels. CRISPR interference (CRISPRi) has been proven to be the most effective and specific tool to knock down any gene of interest in mammalian cells. The catalytically deactivated Cas9 (dCas9) can be fused with transcription repressors to downregulate gene expression specified by sgRNA complementary to target genomic sequence. Although CRISPRi has huge potential for gene knockdown, there is still a lack of (...) systematic guidelines for efficient and widespread use. Here we describe the working mechanism and development of CRISPRi, designing principles of sgRNA, delivery methods and applications in mammalian cells in detail. Finally, we propose possible solutions and future directions with regard to current challenges. (shrink)

Based on the social identity theory, the relationship and influencing mechanism between high-performance human resource practices and employees’ voice behavior were explored by constructing a moderated mediation model, and the relationship between the field of human resources and the field of organizational behavior was also established. Through 1,178 paired samples of supervisor-employee survey and multilevel linear model analysis technology, it was found that HPHRPs had a positive impact on employees’ voice behavior; perceived insider status played a mediating role between HPHRPs (...) and voice behavior; voice efficacy played a moderating role between perceived insider status and voice behavior; and voice efficacy played a mediating role in the relationship between “HPHRPs-perceived insider status-voice behavior.”. (shrink)

This research examines how and why reactions to other consumers’ unethical behavior differ among consumers and vary in different situations. Drawing on construal level theory, the authors propose that the relationship between other consumers’ unethical behavior and focal consumers’ unethical behavior is moderated by focal consumers’ construal level, and self-expressiveness mediates this moderating effect. Specifically, consumers at higher construal levels tend to view their behavior as more self-expressive and are thus less likely to imitate other consumers’ unethical behavior. Study 1 (...) indicates that focal consumers who have a chronic tendency to construe information at high levels are less likely to replicate other consumers’ unethical behavior than those who have a chronic tendency to construe information at low levels. Studies 2, 3a and 3b show that when primed with higher-level construals, consumers are less likely to follow other consumers’ unethical behavior than when primed with lower-level construals. The mediating effect of self-expressiveness was tested and supported in Study 1 and Study 3b. These findings help to better understand consumers’ reactions to other consumers’ unethical behavior. (shrink)

ObjectiveTwo subregions of the dorsolateral prefrontal cortex have been identified as effective repetitive transcranial magnetic stimulation targets for the “anxiosomatic” and “dysphoric” symptoms, respectively. We aimed to develop a convenient approach to locate these targets on the scalp.Materials and methodsIn a discovery experiment, the two personalized targets were precisely identified on 24 subjects using a neuronavigation system. Then, a localized approach was developed based on individual scalp landmarks. This “landmark-based approach” was replicated and validated in an independent cohort. Reliability of (...) the approach was tested by calculating the correlation of both the inter-rater and intra-rater results. Validity was tested by comparing the mean distance between the personalized and landmark-based targets to the TMS spatial resolution. We further conducted a total of 24 sham rTMS sessions to estimate the misplacement between the coil center and target during a 10-min stimulation without neuronavigation.ResultsThe parameters of the “landmark-based approach” in the discovery experiment were replicated well in an independent cohort. Using discovery parameters, we successfully identified the symptom-specific targets in the independent cohort. Specifically, the mean distance between the personalized and landmark-based targets on the cortex was not significantly larger than 5 mm. However, the personalized and landmark-based targets distance exceeded 5 mm in more than 50% of subjects. During the 10-min sham rTMS session, the average coil misplacement was significantly larger than 5 mm.ConclusionThe “landmark-based approach” can conveniently and reliably locate the two symptom-specific targets at group level. However, the accuracy was highly varied at individual level and further improvement is needed. (shrink)

Interactions between human beings and chatbots are gradually becoming part of our everyday social lives. It is still unclear how human–chatbot interactions, compared to human–human interactions, influence individual morality. Building on the dual-process theory of moral judgment, a secondary data analysis, and two scenario-based experiments provide sufficient evidence that HCIs support utilitarian judgments, which reduce participants' donation amount. Study 3 further demonstrates that the negative effects of HCIs can be attenuated by inducing a social-oriented communication style in chatbots’ verbal language (...) designs. These findings highlight the negative impacts of HCIs on relationships among human beings and suggest a practical intervention for nonprofit organization managers. (shrink)

ObjectivesSeveral studies have examined the effects of repetitive transcranial magnetic stimulation (rTMS) on associative memory (AM) but findings were inconsistent. Here, we aimed to test whether twice-daily rTMS could significantly improve AM.MethodsIn this single-blind, sham-controlled experiment, 40 participants were randomized to receive twice-daily sham or real rTMS sessions for five consecutive days (a total of 16,000 pulses). The stimulation target in left inferior parietal lobule (IPL) exhibiting peak functional connectivity to the left hippocampus was individually defined for each participant. Participants (...) completed both a picture-cued word association task and Stroop test at baseline and 1 day after the final real or sham rTMS session. Effects of twice-daily rTMS on AM and Stroop test performance were compared using two-way repeated measures analysis of variance with main factors Group (real vs. sham) and Time (baseline vs. post-rTMS).ResultsThere was a significant Group × Time interaction effect. AM score was significantly enhanced in the twice-daily real group after rTMS, but this difference could not survive the post hoc analysis after multiple comparison correction. Further, AM improvement in the twice-daily real group was not superior to a previously reported once-daily rTMS group receiving 8,000 pulses. Then, we combined the twice- and once-daily real groups, and found a significant Group × Time interaction effect. Post hoc analysis indicated that the AM score was significantly enhanced in the real group after multiple comparisons correction.ConclusionOur prospective experiment did not show significant rTMS effect on AM, but this effect may become significant if more participants could be recruited as revealed by our retrospective analysis. (shrink)

Three hundred and seventy-six middle school students in Wenchuan County were assessed three and one-half years after the Wenchuan earthquake to examine the effects of rumination on posttraumatic stress disorder (PTSD) and posttraumatic growth (PTG). The results revealed that recent intrusive ruminations partly mediated the relationship between intrusive rumination soon after the earthquake with PTSD but not with PTG. Recent deliberate rumination partly mediated the relationship between intrusive rumination soon after the earthquake and PTG but not PTSD. Moreover, recent deliberate (...) rumination also partly mediated the relationship between recent intrusive rumination with PTG but not with PTSD. Overall, intrusive rumination soon after the earthquake had an effect on PTSD but not on PTG through recent intrusive rumination and affected PTG but not PTSD through deliberate recent rumination. Furthermore, intrusive rumination soon after the earthquake affected PTG but not PTSD by recent deliberate rumination following recent intrusive rumination. More importantly, the present study also found that PTSD exhibited no relation to PTG. These results suggest that PTSD and PTG are influenced by different mechanisms, which further indicates that PTSD and PTG represent two separate dimensions of experience after adversity. (shrink)

Since learners’ academic achievement is the ultimate goal of any system of education, multitudes of studies have been conducted on this variable and its determinants. That is, several scholars have explored the effects of student-related and teacher-related factors on learners’ academic achievement. Notwithstanding, a few researchers have investigated the effects of teachers’ self-efficacy and creativity on learners’ academic achievement. Furthermore, no study has reviewed the role of these teacher-related factors in learners’ achievement. Therefore, the present study seeks to bridge the (...) lacunas by delving into the influence of teachers’ self-efficacy and creativity on English as a Foreign Language learners’ academic achievement. The beneficial effects of self-efficacy beliefs and creative teaching were outlined through the existing evidence. This seems to be illuminative for EFL teachers. Given the positive consequences of teachers’ self-efficacy for students’ achievement, EFL teachers should believe in themselves and their abilities to lead their students to academic success. Additionally, with regard to the positive impact of teachers’ creativity on learners’ achievement, EFL teachers are expected to teach the course content creatively. (shrink)

The findings of this article reveal that the development of the independent learning model in the American literature curriculum positively impacts teachers’ job psychological satisfaction. This study highlights the contribution of autonomous learning mode in empowering students’ subjective status and enhancing their initiative, consequently reducing teachers’ psychological pressure and improving their overall satisfaction with their work. Contribution: The results of this study hold implications for scholars in the field, particularly those engaged in practical theology and religious educational studies.

Bankruptcy of listed companies or shareholders delisting usually causes the crisis spreading in stock markets. Based on the systematic analysis of the epidemic diseases and rumors spreading on the complex networks, the SIR model is introduced to research the crisis spreading in shareholding networks of listed companies and their main holders on the basis of the data about ownership structure in Chinese Stock Markets. The characteristics of shareholding networks are studied, and the parameters for the SIR model are obtained by (...) empirical approach. Then, the numerical computation method is successfully used to analyze the crisis spreading in the networks when the networks meet random failures or intentional attacks. We find the networks have good robustness against the random failures. However, the crisis will spread at a high speed and cause catastrophic damage if there are some failures or attacks on hub vertices in the networks. Under this condition, the networks show obvious vulnerability. Last but not least, the controllability of the networks under the condition of intentional attacks and random failures is studied. The results show that if the network is controlled globally, it is more reliable to allow a politically good new or an appropriate exciting economical policy to play the role in orienting markets under the control of public opinions as the crisis occurs. However, under normal circumstances, controlling a small part of driver vertices representing listed companies, applying appropriate control strategies, and using its characteristics of high efficiency of sending information can effectively control the stock market. Our research provides a new reference to further exploration about the transmission mechanism of the crisis based SIR model and further research on the controllability of crisis spreading in financial markets. (shrink)

As patient autonomy expands, a highly controversial issue has emerged. Should the advance directives of refusing life-saving treatments or requesting euthanasia of persons with dementia who express changed minds or are often in a happy state be fulfilled? There are two autonomy-related positions. The mainstream position in philosophical discussions supports the priority of ADs based on precedent autonomy. Buchanan and Brock, and Dworkin represent this view. The other position supports the priority of PWDs’ current wishes based on minimal autonomy represented (...) by Jaworska. By rethinking the theoretical and practical challenges of the two positions and their arguments respectively, the paper concludes that in such a scenario, the priority of ADs is morally indefensible, and it is also challenging to establish minimal autonomy defend the moral priority of PWDs’ current wishes. For PWDs whose past values, beliefs, and preferences fade away but who retain apparent intrinsic sentience of well-being, a modified defined quality-of-life approach serves as another reasonable basis in their medical decision-making, and the ADs and the QoL should be further merged to reduce the uncertainty of the grey zone. (shrink)

Many moral error theorists reject moral realism on the grounds that moral realism implies the existence of categorical normativity, yet categorical normativity does not exist. Call this the Metaphysical Argument. In response, some moral realists have emphasized a parity between moral normativity and epistemic normativity. They argue that if one kind of normativity is rejected, then both must be rejected. Therefore, one cannot be a moral error theorist without also being an epistemic error theorist. Call this the Parity Argument. In (...) this paper, we address three possible responses to the Parity Argument from moral error theorists: (1) accept the parity but still reject epistemic error theory, (2) reject the parity, (3) accept the parity and defend epistemic error theory. We argue that there are problems with each of these responses, so the Parity Argument stands as a strong counterargument to the Metaphysical Argument. We conclude by drawing some lessons for any future challenges to the Parity Argument. (shrink)

The heterogeneity of transcranial direct current stimulation protocols and clinical profiles may explain variable results in modulating excitability in the motor cortex after stroke. However, the cortical electrical effects induced by different tDCS protocols remain unclear. Here, we aimed to compare rhythm changes in electroencephalography induced by three tDCS position protocols and the association between tDCS effects and clinical factors in stroke. Nineteen patients with chronic ischemic stroke underwent four experimental sessions with three tDCS protocols [anodal, cathodal, and bilateral ] (...) and a sham protocol, according to a single-blind randomized crossover design. Resting-state EEG was acquired before and after each protocol. First, a paired-sample t-test was used to examine the difference in spectral power between pre- and post-stimulation. Then, linear and quadratic regression models were used separately to describe the association between the clinical factors of stroke and changes in spectral power which was significantly different between pre- and post-tDCS. Finally, repeated measures analysis of variance with lesion hemisphere, stimulation protocol, and the location was performed to investigate the effects of tDCS over time. The induced effect of tDCS was mainly reflected in the alpha rhythms. The alpha power was increased by atDCS, especially low-alpha, in localized areas of the central and distant areas of the frontal and parietal lobes. Bi-tDCS also affected alpha power but in a smaller area that mainly focused on high-alpha rhythms. However, ctDCS and sham had no significant effects on any EEG rhythm. The clinical factors of time since stroke and motor impairment level were related to the change in high-alpha induced by atDCS and bi-tDCS following quadratic regression models. The above-mentioned modulation effect lasted for 20 min without attenuation. In conclusion, our findings provide evidence that the alpha rhythm of EEG is modulated differently by different tDCS protocols and that high alpha is affected by clinical characteristics such as post-stroke time and motor deficits, which is of great significance for understanding the modulation effect of different tDCS protocols on stroke and the guidance of protocols to promote motor recovery following stroke. (shrink)

The competition between government-provided apps and business-provided apps for active users in China is becoming increasingly fierce. Apps with higher user activity will win this competition. To maintain active users, finding the user activity influencing factors is crucial. In this study, we selected a government-provided app—“Beijing One Card”- and a business-provided app—“Bus Code” -in the field of public transportation as the comparative research objects. Based on multiple regression analysis, we explored the factors influencing user activity. We found user preference plays (...) a critical role in distinguishing the influencing factors of user activity between two kinds of apps. Distinct from the existing research, the service quality does not affect the user activity of public transportation apps. This paper argues that, whether it is a government-provided app or a business-provided app, to enhance the user activity of the app, in the short term, it is necessary to improve the app function according to the user’s needs, try to provide services which user like, cater to user’s preference, strengthen user experience; In the long run, it is essential to pay attention to mining the user’s data accumulated while operating the app to understand user behavior. So as to affect the user’s preference, enhance user’s trust, and improve user participation through public policy. (shrink)

周山(1949-),上海社会科学院哲学研究所副研究员、周易研究中心秘书长、中国逻辑史研究会理事、上海市逻辑学会理事.

Cooley's argument that persons with pre‐dementia have a Kantian duty to die has led to much debate. Cooley gives two reasons for his claim, the first being that a person with pre‐dementia should end his/her life when he/she will inevitably and irreversibly lose rationality and be unable to live morally as a result. This paper argues that this reason derives from an unsubstantiated premise and general confusion regarding the condition for a Kantian duty to die. Rather, a close reading of (...) Kant reveals that such a condition occurs when a person confronts an external handicap that does not undermine his/her rational ability but deprives him/her of the possibility of living the way a person should. People do not confront this experience with progressive dementia. The other reason Cooley proposes is that a person should not allow their continued existence to become a burden to others. This claim partly stems from a radical interpretation of a case discussed by Kant and is partly based on a misuse of Kant's formulation of humanity. Based on a prudent inference from Kantian ethics, this article argues against Cooley that persons with pre‐dementia have no Kantian duty to die. (shrink)

This commentary focuses on two issues. First, reflecting on the distinction between public and private institutions in protecting individual rights is necessary. Second, the current regulatory appr...

Generally believed that "theory of continuing revolution under the dictatorship of the proletariat" is a "Cultural Revolution" guiding ideology, which in ten years the "Cultural Revolution" period has been widely publicized, has written to the Chinese Communists "nine", "Top Ten", "Eleventh large "by the political report and constitution, but also to write the fourth and fifth National People's Congress meeting adopted constitutional changes, the impact is huge. When it was proposed that "the history of Marxism has set a third great (...) milestone," and after more than ten years later, "since the founding of the party on a number of resolutions on the question of history" has made ​​it a completely different assessment that It is the "leftist" errors and this should be "resolutely corrected." In this paper, the editorial "October Socialist Revolution opened up along the path," CPC "nine", Hua Guofeng, the Third Plenum and "since the founding of the party on a number of resolutions on the question of history," as the coordinates of the tracking the fate of its history and study. q "The Theory on Continuing the Revolution under the Leadership of Proletarians" is commonly regarded as the guiding ideology of the Cultural Revolution. Widely publicized during the decade-long Cultural Revolution, it has been incorporated into the Chinese Communist Party's constitution and the People's Republic of China's constitution for numerous times and has exerted a great influence on the state. By the time it was initially proposed in 1967, it was regarded as' the third landmark in the history of Marxism. But it was subject to a completely different fate more than ten years later as it was labeled "pinko" and was required to he "corrected" in Resolution on Certain Questions in the History of Our Party since the Founding of the People's Republic of China. This paper aims to conduct an investigation of the historical fate of "the Theory on Continuing the Revolution under the Dictatorship of Proletarians" with reference to the following texts: Go Ahead Along the Way Inaugurated by October Socialist Revolution, Report of the Ninth National Congress of the CPC, Hua Guofeng's Talkings, Report of the Third Plenary Session of the 11th Central Committee of the Communist Party of China and Resolution on Certain Questions in the History of Our Party since the Founding of the People's Republic of China. (shrink)

Deception is common in dementia care, although its moral legitimacy is questionable. This paper conceptually clarifies when does dementia care involve deception and argues that care ethics is an appropriate ethical framework to guide dementia care compared with the mainstream ethical theories that emphasize abilities. From a perspective of care ethics, this paper claims that morally defensible deception is context-specific, embodied as a caring process that needs to be identified through instant, creative and interactive care procedures. According to this argument, (...) it further analyses and concludes the moral rationality of deception in five common cases. While deception is morally justified in many situations, another concern is that it is usually not the last resort but a means of convenience. As the ability to interact, autonomy and dignity of persons with dementia (PWDs) are generally ignored; nurses need more training and education to master the essential procedures to ensure that the value of authenticity and autonomy is maintained and considered throughout dementia care. (shrink)

There is an important contemporary debate in moral responsibility about whether the following asymmetry thesis is true: moral responsibility for actions does not require alternative possibilities but moral responsibility for omissions does. In this paper, we do two things. First, we consider and reject a recent argument against the asymmetry thesis, contending that the argument fails because it rests on a false view about the metaphysics of omissions. Second, we develop and defend a new argument against the asymmetry thesis, one (...) that avoids the problem with the first argument by not resting on any assumptions about what omissions are metaphysically. (shrink)

The Dao De Jing (Tao Te Ching) by Laozi (Lao Tzu) is unquestionably the best known and most widely read of all classical Chinese philosophy texts. It has been adapted into all forms of modern media, including business, personal relationships, meditation, and sports. Rarely has a book been published containing the essential, unaltered text in an aesthetically minimalist format, preserving the essence of the original prose. Like the writings of Sunzi, Confucius, and Mencius, the work of Laozi also forms one (...) of the founding pillars of the Chinese artistic and literary canon. The Dao De Jing offers profound insight into classical Chinese philosophical thought, while providing practical, sensible advice for leading ones life in today's modern world. A Basic Dao joins the other titles in the Long River Press' Basic series, which highlights the best examples of ancient Chinese philosophy in their pure, unaltered forms. A Basic Dao takes some of the most popular quotations from Laozi and arranges them with historical anecdotes on the opposite page. The result is an ideal book for beginning or experienced philosophy students, philanthropists, and anyone with an interest in the philosophical and literary traditions of China. (shrink)

本书致力于进行论述努力具备“和”之美德,把握“和”的规律,从而追求与他人、事业的和谐,达到“和气生财”的目的。.

本书对早期中国经典中的身体观,作了思想与修行相交融的通盘观照;并在两希传统和印度传统身体观的考察基础上,展开跨文化比较与对话。.

本书涵盖了国家教委《法理学教学指导纲要》规定的知识要点,同时增加了近年我国法理学研究的新成果以及西方法理学中的经典课题。.

This article provides a comprehensive conceptual analysis of information. It begins with a folk notion that information is a tripartite phenomenon: information is something carried by signals about something for some use. This suggests that information has three main aspects: structural, referential, and normative. I analyze the individually necessary and jointly sufficient conditions for defining these aspects of information and consider formal theories relating to each aspect as well. The analysis reveals that structural, referential, and normative aspects of information are (...) hierarchically nested and that the normative depends on the referential, which in turn depends on the structural. (shrink)

This thesis is about the creativity in the East and the West, but I will mainly focus on the view of creativity in ancient Greek philosophy and Chinese philosophy. In the first chapter, I will explore the concept of creativity, the history of creativity, and the research on creativity, including the creativity research in psychology and philosophy, which will set the stage for further disscusion. Then in the second chapter, I will start from Plato’s dialogue, Ion, and explore the traditional (...) theory of creativity in ancient Greek philosophy, and claim that their creative ability is inspired by divine being. After this, I will show people’s view on creativity has undergone a process of demystification. During this process, people gradually began to resist the divine source and explore the world individually and independently. Meanwhile, the meaning of the concept of creativity has become broader, since it is not limited to poem any more, but it can be applied to other fields, such as art, science, and education. In the third chapter, I will turn to the modern creativity theory, including the lateral thinking and emotional creativity, and explore how to be creative with lateral thinking techniques and how to improve creativity through emotion regulation. After the examination in the West, I will turn to the theory of creativity in Chinese philosophy, by focusing on classical works in Daoism and Confucianism. In the fourth chapter, I will explore the view of creativity in philosophy of Daoism. I will claim that the creativity in Daoism is natural and cosmological, and there is no external creative source, so I will claim it is self- creativity, which is distinct from the ancient Western creativity pushed by external divine power. In the fifth chapter, I will argue that the creativity in Confucianism is evolutionary, which is unlike the revolutionary creativity in the West, and I will propose the way to become creative in Confucianism based on its classics, Analects, and then will explore the creativity principle and how the agent cultivate their creative ability in Confucianism. In the conclusion, I will summarize the difference between the creativity in East and West, from the perspective of the source of creativity, nature of creativity. I will claim that the source of creativity in ancient Greek philosophy is divine being, whereas there is no divine source for creativity in Chinese philosophy, therefore, the creativity in the West traditionally is inspired creativity, while the creativity in the East traditionally is natural and cosmological creativity. In addition, the concept of creativity in the West is revolutionary, whereas the concept of creativity in the East is evolutionary and the moral function of creativity is also addressed in Chinese philosophy. (shrink)

Creativity is a developing topic in philosophy in recent years, and it raises a series of challenging questions both in theory and practice for us. In this paper, I will explore creativity with the lateral thinking techniques which aim to solve problems in a creative and lateral way. I will examine the meaning of lateral thinking and its three kinds, the conceptual lateral thinking, the emotive lateral thinking, and the diagrammatic lateral thinking, trying to find out how the space of (...) possible solutions is affected by lateral thinking, which separates the creative from the problem-solver. (shrink)

With the development of economic globalization, more and more clinical research and trials shift from developed countries to developing countries. The globalization of clinical trials also brings some ethical and scientific concerns. This paper discussed some ethical problems of clinical trials in the developing countries including 1) "golden rice" event in China; 2) Informed consent in clinical trials in developing countries; 3) Ethical consciousness in clinical research and trials in developing countries. The essay suggests that the level of medical ethics (...) in developing countries is lower than that in developed countries. How can we improve the ethical consciousness of clinical researchers and subjects and how can we protect patient rights are important problems faced by developing countries. (shrink)

本书主要探讨传统思想与科学发展之间的关系,涉及传统哲学的科学基础、科学发展的思想原因、科学思想的涵义和演变、以及传统思想对数学、医学等各门学科的作用和影响。.