We have developed a first generation tool for the unbiased identification and characterization of human pluripotent stem cells, termed PluriTest. This assay utilizes all the information contained on a microarray and abandons the conventional stem cell marker concept. Stem cells are defined by the ability to replenish themselves and to differentiate into more mature cell types. As differentiation potential is a property that cannot be directly proven in the stem cell state, biologists have to rely on correlative measurements in stem (...) cells associated with differentiation potential. Unfortunately, most, if not all, of those markers are only valid within narrow limits of specific experimental systems. Microarray technologies and recently next‐generation sequencing have revolutionized how cellular phenotypes can be characterized on a systems‐wide level. Here we discuss the challenges PluriTest and similar global assays need to address to fulfill their enormous potential for industrial, diagnostic and therapeutic applications. (shrink)

In many developing countries people and livestock suffer from preventable or curable diseases, and their agriculture is vulnerable to natural disasters. A considerable amount of technical aid is directed at alleviating these problems using modern science and technology, and yet most of these efforts either fail or even leave peasants and pastoralists worse off than before. In this paper we consider some of the problems that arise in relation to development projects, focusing our attention on the savannah regions of Africa (...) and, in particular, on the control of tsetse flies, which are the vectors of the African trypanosomiases, called nagana in cattle and sleeping sickness in people. We present a detailed case study of a project designed to enable a Maasai community in Kenya to carry out their own tsetse fly control. We examine the complex set of relationships and power structures that mediate the actions of the players in development: scientists, local communities, governmental and nongovernmental institutions, and development agencies. The purpose of this paper is not to present solutions to complex and difficult problems but rather to raise questions that should provide a framework for a debate concerning the role of science and technology in the development process. (shrink)