Caffeine, a known CNS stimulant is given as an adjunct component in most abused drugs which could be fatal with repeated administration in many circumstances. This paper presents a study to investigate the effect of repeated administration of caffeine at high dose on rat liver, and discusses ethical and policy issues of caffeine use. Long Evans rats were treated with pure caffeine solution in distilled water through intragastric route once daily for consecutive 56 days. Three groups of rats recognized as (...) low dose, high dose and control group received 6mg caffeine / kg BW, 12mg caffeine / kg BW and distilled water, respectively. Rat plasma was examined for liver transaminases and alkaline phosphatases concentrations which were significantly increased in plasma as compared to the control. Both rat plasma and liver homogenate were subjected to estimate malondialdehyde, advanced oxidation protein product, nitric oxide, antioxidant enzyme catalase, glutathione and superoxide dismutase activity. MDA, AOPP, NO levels increased and SOD activity decreased significantly in both plasma and liver as compared to those of control where as CAT and GSH activity remain unchanged. Rat liver tissues were studied histochemically with Hematoxylin and Eosin, and Picro Sirius Red staining. Significantly increased infiltration of inflammatory cells and progressive deposition of collagen fibre were visible in liver tissue of caffeine treated both dose groups as compared to the control. Long term administration of caffeine at higher dose, significantly contributes to liver inflammation and consequent fibrogenesis. This raises significant ethical and policy issues. (shrink)

This research article offers a study on a new relation of rough sets and soft sets with an algebraic structure quantale module by using soft reflexive and soft compatible relations. The lower approximation and upper approximation of subsets of quantale module are utilized by aftersets and foresets. As a sequel of this relation, different characterizations of rough soft substructures of quantale modules are obtained. To ensure the results, soft reflective and soft compatible relations are focused and these are interpreted by (...) aftersets and foresets. Furthermore, the algebraic relations between upper approximation of substructures of quantale module and the upper approximations of their homomorphic images with the help of quantale module homomorphism are examined. In comparison with the different type of approximations in different type of algebraic structures, it is concluded that this new study is much better. (shrink)