Effect of freezing on the relative escape force of sperm as measured by a laser optical trap

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Abstract

Objective: To determine the possible effect of freezing on sperm escape force as measured by laser trap. Design: Controlled clinical study. Setting: Normal volunteers, academic setting. Patients: Normal, healthy volunteers. Intervention: Sperm selection, freezing and thawing. Measurement of relative escape force before and after freezing. Main Outcome Measure: Comparison of escape force as a measurement of freezing effects. Results: Wide individual variations noted, with some individuals showing significant increases or decreases. The relative escape force in fresh and in frozen-thawed samples were similar. Conclusion: Freezing does not affect the relative escape force of normal sperm. Any possible detrimental effect of freezing on sperm may be related to other sperm functions.

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