Recent years have seen enticing empirical approaches to solving the epistemological problem of the theory-ladenness of observation. I group these approaches in two categories according to their method of choice: testing and refereeing. I argue that none deliver what friends of theory-neutrality want them to. Testing does not work because both evidence from cognitive neuroscience and perceptual pluralism independently invalidate the existence of a common observation core. Refereeing does not work because it treats theory-ladenness as a kind of superficial, removable (...) bias. Even if such treatment is plausible, there is likely no method to ascertain that effects of this bias are not present. More importantly, evidence from cognitive neuroscience suggests that a deeper, likely irremovable kind of theory-ladenness lies within the perceptual modules. (shrink)

Philosophers of science diverge on the question what drives the growth of scientific knowledge. Most of the twentieth century was dominated by the notion that theories propel that growth whereas experiments play secondary roles of operating within the theoretical framework or testing theoretical predictions. New experimentalism, a school of thought pioneered by Ian Hacking in the early 1980s, challenged this view by arguing that theory-free exploratory experimentation may in many cases effectively probe nature and potentially spawn higher evidence-based theories. Because (...) theories are often powerless to envisage workings of complex biological systems, theory-independent experimentation is common in the life sciences. Some such experiments are triggered by compelling observation, others are prompted by innovative techniques or instruments, whereas different investigations query big data to identify regularities and underlying organizing principles. A distinct fourth type of experiments is motivated by a major question. Here I describe two question-guided experimental discoveries in biochemistry: the cyclic adenosine monophosphate mediator of hormone action and the ubiquitin-mediated system of protein degradation. Lacking underlying theories, antecedent data bases, or new techniques, the sole guides of the two discoveries were respective substantial questions. Both research projects were similarly instigated by theory-free exploratory experimentation and continued in alternating phases of results-based interim working hypotheses, their examination by experiment, provisional hypotheses again, and so on. These two cases designate theory-free, question-guided, stepwise biochemical investigations as a distinct subtype of the new experimentalism mode of scientific enquiry. (shrink)