Innovative loss‐of‐function techniques developed in recent years have made it much easier to target specific genomic loci at transcriptional levels. CRISPR interference (CRISPRi) has been proven to be the most effective and specific tool to knock down any gene of interest in mammalian cells. The catalytically deactivated Cas9 (dCas9) can be fused with transcription repressors to downregulate gene expression specified by sgRNA complementary to target genomic sequence. Although CRISPRi has huge potential for gene knockdown, there is still a lack of (...) systematic guidelines for efficient and widespread use. Here we describe the working mechanism and development of CRISPRi, designing principles of sgRNA, delivery methods and applications in mammalian cells in detail. Finally, we propose possible solutions and future directions with regard to current challenges. (shrink)

This research examines how and why reactions to other consumers’ unethical behavior differ among consumers and vary in different situations. Drawing on construal level theory, the authors propose that the relationship between other consumers’ unethical behavior and focal consumers’ unethical behavior is moderated by focal consumers’ construal level, and self-expressiveness mediates this moderating effect. Specifically, consumers at higher construal levels tend to view their behavior as more self-expressive and are thus less likely to imitate other consumers’ unethical behavior. Study 1 (...) indicates that focal consumers who have a chronic tendency to construe information at high levels are less likely to replicate other consumers’ unethical behavior than those who have a chronic tendency to construe information at low levels. Studies 2, 3a and 3b show that when primed with higher-level construals, consumers are less likely to follow other consumers’ unethical behavior than when primed with lower-level construals. The mediating effect of self-expressiveness was tested and supported in Study 1 and Study 3b. These findings help to better understand consumers’ reactions to other consumers’ unethical behavior. (shrink)

Three hundred and seventy-six middle school students in Wenchuan County were assessed three and one-half years after the Wenchuan earthquake to examine the effects of rumination on posttraumatic stress disorder (PTSD) and posttraumatic growth (PTG). The results revealed that recent intrusive ruminations partly mediated the relationship between intrusive rumination soon after the earthquake with PTSD but not with PTG. Recent deliberate rumination partly mediated the relationship between intrusive rumination soon after the earthquake and PTG but not PTSD. Moreover, recent deliberate (...) rumination also partly mediated the relationship between recent intrusive rumination with PTG but not with PTSD. Overall, intrusive rumination soon after the earthquake had an effect on PTSD but not on PTG through recent intrusive rumination and affected PTG but not PTSD through deliberate recent rumination. Furthermore, intrusive rumination soon after the earthquake affected PTG but not PTSD by recent deliberate rumination following recent intrusive rumination. More importantly, the present study also found that PTSD exhibited no relation to PTG. These results suggest that PTSD and PTG are influenced by different mechanisms, which further indicates that PTSD and PTG represent two separate dimensions of experience after adversity. (shrink)

ObjectiveTwo subregions of the dorsolateral prefrontal cortex have been identified as effective repetitive transcranial magnetic stimulation targets for the “anxiosomatic” and “dysphoric” symptoms, respectively. We aimed to develop a convenient approach to locate these targets on the scalp.Materials and methodsIn a discovery experiment, the two personalized targets were precisely identified on 24 subjects using a neuronavigation system. Then, a localized approach was developed based on individual scalp landmarks. This “landmark-based approach” was replicated and validated in an independent cohort. Reliability of (...) the approach was tested by calculating the correlation of both the inter-rater and intra-rater results. Validity was tested by comparing the mean distance between the personalized and landmark-based targets to the TMS spatial resolution. We further conducted a total of 24 sham rTMS sessions to estimate the misplacement between the coil center and target during a 10-min stimulation without neuronavigation.ResultsThe parameters of the “landmark-based approach” in the discovery experiment were replicated well in an independent cohort. Using discovery parameters, we successfully identified the symptom-specific targets in the independent cohort. Specifically, the mean distance between the personalized and landmark-based targets on the cortex was not significantly larger than 5 mm. However, the personalized and landmark-based targets distance exceeded 5 mm in more than 50% of subjects. During the 10-min sham rTMS session, the average coil misplacement was significantly larger than 5 mm.ConclusionThe “landmark-based approach” can conveniently and reliably locate the two symptom-specific targets at group level. However, the accuracy was highly varied at individual level and further improvement is needed. (shrink)

Based on the social identity theory, the relationship and influencing mechanism between high-performance human resource practices and employees’ voice behavior were explored by constructing a moderated mediation model, and the relationship between the field of human resources and the field of organizational behavior was also established. Through 1,178 paired samples of supervisor-employee survey and multilevel linear model analysis technology, it was found that HPHRPs had a positive impact on employees’ voice behavior; perceived insider status played a mediating role between HPHRPs (...) and voice behavior; voice efficacy played a moderating role between perceived insider status and voice behavior; and voice efficacy played a mediating role in the relationship between “HPHRPs-perceived insider status-voice behavior.”. (shrink)

Interactions between human beings and chatbots are gradually becoming part of our everyday social lives. It is still unclear how human–chatbot interactions, compared to human–human interactions, influence individual morality. Building on the dual-process theory of moral judgment, a secondary data analysis, and two scenario-based experiments provide sufficient evidence that HCIs support utilitarian judgments, which reduce participants' donation amount. Study 3 further demonstrates that the negative effects of HCIs can be attenuated by inducing a social-oriented communication style in chatbots’ verbal language (...) designs. These findings highlight the negative impacts of HCIs on relationships among human beings and suggest a practical intervention for nonprofit organization managers. (shrink)

ObjectivesSeveral studies have examined the effects of repetitive transcranial magnetic stimulation (rTMS) on associative memory (AM) but findings were inconsistent. Here, we aimed to test whether twice-daily rTMS could significantly improve AM.MethodsIn this single-blind, sham-controlled experiment, 40 participants were randomized to receive twice-daily sham or real rTMS sessions for five consecutive days (a total of 16,000 pulses). The stimulation target in left inferior parietal lobule (IPL) exhibiting peak functional connectivity to the left hippocampus was individually defined for each participant. Participants (...) completed both a picture-cued word association task and Stroop test at baseline and 1 day after the final real or sham rTMS session. Effects of twice-daily rTMS on AM and Stroop test performance were compared using two-way repeated measures analysis of variance with main factors Group (real vs. sham) and Time (baseline vs. post-rTMS).ResultsThere was a significant Group × Time interaction effect. AM score was significantly enhanced in the twice-daily real group after rTMS, but this difference could not survive the post hoc analysis after multiple comparison correction. Further, AM improvement in the twice-daily real group was not superior to a previously reported once-daily rTMS group receiving 8,000 pulses. Then, we combined the twice- and once-daily real groups, and found a significant Group × Time interaction effect. Post hoc analysis indicated that the AM score was significantly enhanced in the real group after multiple comparisons correction.ConclusionOur prospective experiment did not show significant rTMS effect on AM, but this effect may become significant if more participants could be recruited as revealed by our retrospective analysis. (shrink)

Since learners’ academic achievement is the ultimate goal of any system of education, multitudes of studies have been conducted on this variable and its determinants. That is, several scholars have explored the effects of student-related and teacher-related factors on learners’ academic achievement. Notwithstanding, a few researchers have investigated the effects of teachers’ self-efficacy and creativity on learners’ academic achievement. Furthermore, no study has reviewed the role of these teacher-related factors in learners’ achievement. Therefore, the present study seeks to bridge the (...) lacunas by delving into the influence of teachers’ self-efficacy and creativity on English as a Foreign Language learners’ academic achievement. The beneficial effects of self-efficacy beliefs and creative teaching were outlined through the existing evidence. This seems to be illuminative for EFL teachers. Given the positive consequences of teachers’ self-efficacy for students’ achievement, EFL teachers should believe in themselves and their abilities to lead their students to academic success. Additionally, with regard to the positive impact of teachers’ creativity on learners’ achievement, EFL teachers are expected to teach the course content creatively. (shrink)

As patient autonomy expands, a highly controversial issue has emerged. Should the advance directives of refusing life-saving treatments or requesting euthanasia of persons with dementia who express changed minds or are often in a happy state be fulfilled? There are two autonomy-related positions. The mainstream position in philosophical discussions supports the priority of ADs based on precedent autonomy. Buchanan and Brock, and Dworkin represent this view. The other position supports the priority of PWDs’ current wishes based on minimal autonomy represented (...) by Jaworska. By rethinking the theoretical and practical challenges of the two positions and their arguments respectively, the paper concludes that in such a scenario, the priority of ADs is morally indefensible, and it is also challenging to establish minimal autonomy defend the moral priority of PWDs’ current wishes. For PWDs whose past values, beliefs, and preferences fade away but who retain apparent intrinsic sentience of well-being, a modified defined quality-of-life approach serves as another reasonable basis in their medical decision-making, and the ADs and the QoL should be further merged to reduce the uncertainty of the grey zone. (shrink)

Bankruptcy of listed companies or shareholders delisting usually causes the crisis spreading in stock markets. Based on the systematic analysis of the epidemic diseases and rumors spreading on the complex networks, the SIR model is introduced to research the crisis spreading in shareholding networks of listed companies and their main holders on the basis of the data about ownership structure in Chinese Stock Markets. The characteristics of shareholding networks are studied, and the parameters for the SIR model are obtained by (...) empirical approach. Then, the numerical computation method is successfully used to analyze the crisis spreading in the networks when the networks meet random failures or intentional attacks. We find the networks have good robustness against the random failures. However, the crisis will spread at a high speed and cause catastrophic damage if there are some failures or attacks on hub vertices in the networks. Under this condition, the networks show obvious vulnerability. Last but not least, the controllability of the networks under the condition of intentional attacks and random failures is studied. The results show that if the network is controlled globally, it is more reliable to allow a politically good new or an appropriate exciting economical policy to play the role in orienting markets under the control of public opinions as the crisis occurs. However, under normal circumstances, controlling a small part of driver vertices representing listed companies, applying appropriate control strategies, and using its characteristics of high efficiency of sending information can effectively control the stock market. Our research provides a new reference to further exploration about the transmission mechanism of the crisis based SIR model and further research on the controllability of crisis spreading in financial markets. (shrink)

The findings of this article reveal that the development of the independent learning model in the American literature curriculum positively impacts teachers’ job psychological satisfaction. This study highlights the contribution of autonomous learning mode in empowering students’ subjective status and enhancing their initiative, consequently reducing teachers’ psychological pressure and improving their overall satisfaction with their work. Contribution: The results of this study hold implications for scholars in the field, particularly those engaged in practical theology and religious educational studies.

Cooley's argument that persons with pre‐dementia have a Kantian duty to die has led to much debate. Cooley gives two reasons for his claim, the first being that a person with pre‐dementia should end his/her life when he/she will inevitably and irreversibly lose rationality and be unable to live morally as a result. This paper argues that this reason derives from an unsubstantiated premise and general confusion regarding the condition for a Kantian duty to die. Rather, a close reading of (...) Kant reveals that such a condition occurs when a person confronts an external handicap that does not undermine his/her rational ability but deprives him/her of the possibility of living the way a person should. People do not confront this experience with progressive dementia. The other reason Cooley proposes is that a person should not allow their continued existence to become a burden to others. This claim partly stems from a radical interpretation of a case discussed by Kant and is partly based on a misuse of Kant's formulation of humanity. Based on a prudent inference from Kantian ethics, this article argues against Cooley that persons with pre‐dementia have no Kantian duty to die. (shrink)

Unprecedented times compel new ways to explore relationships. Using interviews with dating app users quarantined in American cities at the height of the Covid-19 pandemic, I show the impacts of digital mediation on the highly scripted interactional patterns in dating. Drawing from the literature on creative action, temporality, digital affordance, and the materiality of cultural objects, I examine how actors access the creative opportunities in digitally mediated interaction. I find that dating partners creatively mobilized the affordances of digital technologies to (...) approximate a dating script in organizing online interactions, which simultaneously allowed them to formulate relational narratives with varying deviation from the scripted temporal structure. I identify three aspects of digital affordances offering creative opportunities: the dissolution of spatial-temporal boundaries, the production and circulation of digital objects, and the connection between contexts. Taking advantage of an extraordinary moment of cultural and interactional rupture that necessitated digital solutions to connect, this article demonstrates how digital technologies unsettle culturally institutionalized interactional patterns and the formation of meaningful relationships. (shrink)

The competition between government-provided apps and business-provided apps for active users in China is becoming increasingly fierce. Apps with higher user activity will win this competition. To maintain active users, finding the user activity influencing factors is crucial. In this study, we selected a government-provided app—“Beijing One Card”- and a business-provided app—“Bus Code” -in the field of public transportation as the comparative research objects. Based on multiple regression analysis, we explored the factors influencing user activity. We found user preference plays (...) a critical role in distinguishing the influencing factors of user activity between two kinds of apps. Distinct from the existing research, the service quality does not affect the user activity of public transportation apps. This paper argues that, whether it is a government-provided app or a business-provided app, to enhance the user activity of the app, in the short term, it is necessary to improve the app function according to the user’s needs, try to provide services which user like, cater to user’s preference, strengthen user experience; In the long run, it is essential to pay attention to mining the user’s data accumulated while operating the app to understand user behavior. So as to affect the user’s preference, enhance user’s trust, and improve user participation through public policy. (shrink)

This commentary focuses on two issues. First, reflecting on the distinction between public and private institutions in protecting individual rights is necessary. Second, the current regulatory appr...

Deception is common in dementia care, although its moral legitimacy is questionable. This paper conceptually clarifies when does dementia care involve deception and argues that care ethics is an appropriate ethical framework to guide dementia care compared with the mainstream ethical theories that emphasize abilities. From a perspective of care ethics, this paper claims that morally defensible deception is context-specific, embodied as a caring process that needs to be identified through instant, creative and interactive care procedures. According to this argument, (...) it further analyses and concludes the moral rationality of deception in five common cases. While deception is morally justified in many situations, another concern is that it is usually not the last resort but a means of convenience. As the ability to interact, autonomy and dignity of persons with dementia (PWDs) are generally ignored; nurses need more training and education to master the essential procedures to ensure that the value of authenticity and autonomy is maintained and considered throughout dementia care. (shrink)

There is an important contemporary debate in moral responsibility about whether the following asymmetry thesis is true: moral responsibility for actions does not require alternative possibilities but moral responsibility for omissions does. In this paper, we do two things. First, we consider and reject a recent argument against the asymmetry thesis, contending that the argument fails because it rests on a false view about the metaphysics of omissions. Second, we develop and defend a new argument against the asymmetry thesis, one (...) that avoids the problem with the first argument by not resting on any assumptions about what omissions are metaphysically. (shrink)

The heterogeneity of transcranial direct current stimulation protocols and clinical profiles may explain variable results in modulating excitability in the motor cortex after stroke. However, the cortical electrical effects induced by different tDCS protocols remain unclear. Here, we aimed to compare rhythm changes in electroencephalography induced by three tDCS position protocols and the association between tDCS effects and clinical factors in stroke. Nineteen patients with chronic ischemic stroke underwent four experimental sessions with three tDCS protocols [anodal, cathodal, and bilateral ] (...) and a sham protocol, according to a single-blind randomized crossover design. Resting-state EEG was acquired before and after each protocol. First, a paired-sample t-test was used to examine the difference in spectral power between pre- and post-stimulation. Then, linear and quadratic regression models were used separately to describe the association between the clinical factors of stroke and changes in spectral power which was significantly different between pre- and post-tDCS. Finally, repeated measures analysis of variance with lesion hemisphere, stimulation protocol, and the location was performed to investigate the effects of tDCS over time. The induced effect of tDCS was mainly reflected in the alpha rhythms. The alpha power was increased by atDCS, especially low-alpha, in localized areas of the central and distant areas of the frontal and parietal lobes. Bi-tDCS also affected alpha power but in a smaller area that mainly focused on high-alpha rhythms. However, ctDCS and sham had no significant effects on any EEG rhythm. The clinical factors of time since stroke and motor impairment level were related to the change in high-alpha induced by atDCS and bi-tDCS following quadratic regression models. The above-mentioned modulation effect lasted for 20 min without attenuation. In conclusion, our findings provide evidence that the alpha rhythm of EEG is modulated differently by different tDCS protocols and that high alpha is affected by clinical characteristics such as post-stroke time and motor deficits, which is of great significance for understanding the modulation effect of different tDCS protocols on stroke and the guidance of protocols to promote motor recovery following stroke. (shrink)

La Chine contemporaine 'pense-t-elle'? Et si oui, comment pense-t-elle? Et d'ailleurs, quel sens donner au terme 'penser'? Le noeud du problème réside dans la langue: si la Chine contemporaine veut avoir une pensée authentique, elle doit commencer par étudier sa propre langue? et c'est précisément la difficulté à laquelle se heurte la construction d'un corps intellectuel en chinois. Afin d'éclairer le raisonnement chinois moderne, et de démontrer les implications méthodologiques de la pratique philosophique en chinois, l'auteure a choisi de centrer (...) son étude sur le philosophe chinois contemporain (né en 1952) Chen Jiaying. À la croisée de la linguistique et de la philosophie du langage, elle s'attache à l'approche linguistique de ce philosophe, en particulier à sa manière de développer son raisonnement en clarifiant systématiquement l'usage des mots chinois, et à son style argumentatif et dialogique fondé sur des caractéristiques linguistiques propres à la langue chinoise, mais aussi à travers l'identification et la classification d'une série de concepts chinois 'naturels'. Cet ouvrage démontre ainsi comment la pensée en langue chinoise peut se révéler innovante et dynamique en répondant à des questions universelles cruciales? 'Qu'est-ce que la philosophie aujourd'hui, dans un monde dominé par les méthodes scientifiques empiriques?' 'Qu'est-ce qu'une bonne vie?', 'Comment bien vivre dans notre monde actuel aux valeurs plurielles, fragmentées, voire contradictoires?'...Par-delà l'analyse de ce qu'est 'penser en chinois', Xiyin Zhou ménage une large place à l'analyse conceptuelle interlinguistique, faisant des allers-retours entre les langues chinoise et occidentales. Elle plaide ainsi pour surmonter l'étroitesse de la pratique philosophique dans une seule langue, et pour une approche philosophique 'écolinguistique' fondée sur l'utilisation naturelle du langage."--Page 4 of cover. (shrink)

本书除导论外分为上中下三篇.上篇是法规范论,主要内容有法律的概念,法律的功能和作用,法律关系,法律责任,法律规范,法律渊源和法律分类,法律效力等,是关于静态的法;中篇为法运行论,主要内容有法律程序,立 法,守法,执法,司法,法律方法,法治和法律价值等,是关于动态的法;下篇为法社会论,主要内容有法律与人性,法律与经济,法律与科学,法律与宗教,法律与道德,法律与国家等,是关于法律与其他社会现象的关系.

《滄桑回顧錄》是作者從山溝裡的家鄉為了保家，隨時勢演變而浪跡大江南北，流落海角一隅，終至落葉而無根可歸的人生旅程；也是自十九世紀以來上億臉龐模糊但血淚分明的中國小民從身不由己的經歷與視野所述說的近代史 。對照中國近代官史，《滄桑回顧錄》是人民的歷史。.

The rise of global civil society as a new discourse in the last century, in the new century has been of concern. Western academic circles around the concept of global civil society, global civil society and the emergence and development of global civil society influence on the political realities and trends and other issues had extensive discussions and the formation of a different theoretical framework and research camp. While the Western world made ​​a series of civil society, research results, but (...) still insufficient to demonstrate the normative study, mainly confined to the liberal democratic framework of the inadequacies of the middle. China scholars have been from international relations, political science and political philosophy point of view about global civil society, but in general, the power of research in this area is still relatively weak, insufficient results, further research needs to be made. As a word rising from the late of last century, Global Civil Society is very attractable in the early new century. Western academy has initiated abroad discussion on the concept of GCS, the process of its formation and development, its impact on political realities and its future. In the discussion, theory analysis frames and theory camps have already come into being. Though a great achievements have been gotten, there are still some deficiencies lie in the Western studies, such as lacking of normative studies and main studies are limited in the frames of liberal democracy. In China some scholars have studied about GCS from the views of international relationship, political science and political philosophy. But as a whole, research force in the realm is still weak and the fruits are insufficient, deep discussion is needed. (shrink)

本书第一编主要讨论刑法客观主义理论中所蕴含的抽象性、行为事实的客观性问题及其与法治立场的契合性。第二编主要研究刑法客观主义的反对派即刑法主观主义理论对法治立场的动摇。.